CH02b12
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| CH02b12.F | GGCAGGCTTTACGATTATGC | 20 nt | 58.4 °C |  |
|
| CH02b12.R | CCCACTAAAAGTTCACAGGC | 20 nt | 58.4 °C | ||
| SSR info | |||||
| SSR repeat type | Imperfect | ||||
| Locus type | presumed multi-locus | ||||
| Detected loci | |||||
| Alleles size range | 101-143 | ||||
| Number of alleles detected | 13 | ||||
| Expected heterozygosity | n.d. | ||||
| PCR annealing temp | 60 | ||||
| Sequenced allele size | 139 | ||||
| Linkage group(s) | |||||
| Developed by | ETH Zuerich | ||||
| Other maps | Discovery-5 Discovery x TN10-8-5 Discovery-5 Fiesta-5 Fiesta-10 Fiesta x Totem-5 Apple Integrated Map-5 | ||||
| Reference publication | Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241 | ||||
| Remarks | none | ||||
| Sequence | |||||
| >CH02b12 aattcgacgagcaggcanntgaccttctcgaagagaagaaatgggttgcttaagaaggctaaggagctatcaatcctttg tgatgctgaggttggattgattgtcttctctagcaccggcaggctttacgattatgctagcactaggttagatctctctc tctctctctctctctctctctctctctctctctctttctctctctcaatcaatctatctatatatgtgtgtatattgcct gtgaacttttagtggggtngagtttgcaatgaaactagagctacttgttcgatatagacacagaatatatatgctgcatg ttctagcaaaatatttgaggtagacttgttatattgtactaatt | |||||
| User's notes | |||||
| THERE ARE NO USER'S NOTES. | |||||
| Allele size and gel image | |||||
| Cultivar | Allele size |  | |||
|
Prima Fiesta Discovery Durello di Forlí TN10-8 Florina Nova Easygro Starking |
103:111:129:141 111:127:129 101:109:127:139 125:131 127:141 129:141 141:143 |
||||
Send comments to Luca Gianfranceschi
