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CH02c02a
Primer features
Name Sequence Length Tm  
CH02c02a.F CTTCAAGTTCAGCATCAAGACAA 23 nt 59.3 °C Primer Stats
CH02c02a.R TAGGGCACACTTGCTGGTC 19 nt 59.5 °C
SSR info
SSR repeat type Perfect
Locus type single locus
Detected loci
Alleles size range 129-176
Number of alleles detected 9
Expected heterozygosity 0.88
PCR annealing temp 60
Sequenced allele size 194
Linkage group(s)
Developed by ETH Zuerich
Other maps Discovery-2 Regia x Pingo-2 Regia x Piflora-2 Discovery-2 Fiesta-2 Fiesta x Totem-2 Apple Integrated Map-2
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH02c02a aattgagggtgttagttagggttttaaagatttgttgttggcgggttttattccgaccgattggagttgatgaagcatga tacaaaaaacgaatcttcaagttcagcatcaagacaatactagtagtaaacttagagagagagagagagagaagagagag agagagagagagagaggaggagagagagagagagagagagagagagagagagagtatcaaacatctgtgagattctggag ggatttcaaacttctaaaaccctaatcaggaccagcaagtgtgccctactaat
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
170
148:160
170:172

148:166
n.d.
134:166
129:136


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