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CH02g01
Primer features
Name Sequence Length Tm  
CH02g01.F GATGACGTCGGCAGGTAAAG 20 nt 60.5 °C Primer Stats
CH02g01.R CAACCAACAGCTCTGCAATC 20 nt 58.4 °C
SSR info
SSR repeat type Perfect
Locus type presumed single locus
Detected loci
Alleles size range 198-238
Number of alleles detected 5
Expected heterozygosity 0.7
PCR annealing temp 60
Sequenced allele size 216
Linkage group(s)
Developed by ETH Zuerich
Other maps Fiesta x Totem-13 PI 613988-13 Apple Integrated Map-13 M.27 x M.116-13
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH02g01 aattatgtggatgacgtcggcaggtaaagtttcagactttaatggtagattgcagtctctctctctctctctctctctct ctctctctctctctctctctctcaaatgtatgtttgcattgtgcagtgaatgggatcagcctcctagtgaaatgagacca gctggttcagtttccatcanggtgcgtatttgggttcttaagtttgattgcagagctgttggttggcatgagtttaatgt acgtgtggatttncaggactatgcaatagagacacccttgaaggaatcgttgtcgccggtgggaggcgatacatctcacg aagtgaagattgatgttacacccaacagcgcaatcgcagcgataaatt
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
226:238
226:226
198:218

218:226
218
218:238
218:226


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