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CH03a08
Primer features
Name Sequence Length Tm  
CH03a08.F TTGGTTTGCTAGGAAAAGAAGG 22 nt 58.4 °C Primer Stats
CH03a08.R AAGTTTATCGGGCCTACACG 20 nt 58.4 °C
SSR info
SSR repeat type Imperfect
Locus type single locus
Detected loci
Alleles size range 146-218
Number of alleles detected 7
Expected heterozygosity 0.75
PCR annealing temp 60
Sequenced allele size 185
Linkage group(s)
Developed by ETH Zuerich
Other maps Discovery-13 Fiesta-13 Malling9-13 Robusta5-13 Apple Integrated Map-13
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH03a08 aattcaacttctcacttcattaaatgcatgtctggctggattatcacgtgaacttaaggccttagggtttatttccttcc taactagattggttggtttgctaggaaaagaaggaaacaaggttctctttctccctctctctctctctctctctctctct ctctctctctctcaaacttactcatgtggacttcaaagattatgggcttggatctttaggcccccaancaacccaaagtc ttccataatcttgactccgtgtaggcccgataaacttgtaaccatccacatcacaatt
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
146:186
186:218
164:172

146:192
146:186
146:186
186


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