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CH03a09
Primer features
Name Sequence Length Tm  
CH03a09.F GCCAGGTGTGACTCCTTCTC 20 nt 62.5 °C Primer Stats
CH03a09.R CTGCAGCTGCTGAAACTGG 19 nt 59.5 °C
SSR info
SSR repeat type Perfect
Locus type single locus
Detected loci
Alleles size range 125-143
Number of alleles detected 6
Expected heterozygosity 0.76
PCR annealing temp 60
Sequenced allele size 143
Linkage group(s)
Developed by ETH Zuerich
Other maps Discovery x TN10-8-5 Discovery-5 Fiesta-5 Fiesta x Totem-5 Malling9-5 Robusta5-5 Apple Integrated Map-5 M.27 x M.116-5
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH03a09 aattgtttccctttatattttcatccacccgtggtagtgtgaaatccatctccagagctctcaccttcactctgtttcca tggtctggtctggatttaatccccaaacaaatctttgaaatccaacctcaaccaatcagattcaaccaaactgccaggtg tgactccttctccaccaataccattaaaaactggccagatgatattccaacaganaaaaacctctctctctctctctctc tctctctctctctctctctctctccaaaaaaccatgccagtttcagcagctgcagctctgcantcatctctctgcttctc ttctaacaatccatcatcttcttctgatttggacacccaactggncgcaaaacccaaaagcctgctccacaancatcctc tctacacacccacccacannaaactctccctccaatt
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
131:135
125:131
131:135

135:143
131:143
139:143
131:135


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