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CH03d07
Primer features
Name Sequence Length Tm  
CH03d07.F CAAATCAATGCAAAACTGTCA 21 nt 53.5 °C Primer Stats
CH03d07.R GGCTTCTGGCCATGATTTTA 20 nt 56.4 °C
SSR info
SSR repeat type Perfect
Locus type single locus
Detected loci
Alleles size range 186-226
Number of alleles detected 8
Expected heterozygosity 0.8
PCR annealing temp 60
Sequenced allele size 201
Linkage group(s)
Developed by ETH Zuerich
Other maps Fiesta-6 Discovery-6 Fiesta-6 Fiesta x Totem-6 PI 613988-6 Royal Gala-6 Robusta5-6 Apple Integrated Map-6 M.27 x M.116-6
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH03d07 aattcaaatcaatgcaaaactgtcattgacaatatgtactctctctctctctctctctctctctctctctctctctctat ttagtaactaggcagtctanaacaccgagctgaatgcttactggtcaatgtacacagtttggtcaatgtacacagtttga tttgataagtcccctagttatggaataaaatcatggccagaagccccattttctcaataaatattcttaacaaactccac gggtggcatgcacaaaatctagaaagtttaccagtgaatcaatt
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
206:218
206:218
186:206

206:216
190:202
192:206
206:226


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