CH03g07
Primer features | |||||
Name | Sequence | Length | Tm | ||
CH03g07.F | AATAAGCATTCAAAGCAATCCG | 22 nt | 56.5 °C | ||
CH03g07.R | TTTTTCCAAATCGAGTTTCGTT | 22 nt | 54.7 °C | ||
SSR info | |||||
SSR repeat type | Perfect | ||||
Locus type | single locus | ||||
Detected loci | |||||
Alleles size range | 119-171 | ||||
Number of alleles detected | 5 | ||||
Expected heterozygosity | 0.77 | ||||
PCR annealing temp | 60 | ||||
Sequenced allele size | 123 | ||||
Linkage group(s) | |||||
Developed by | ETH Zuerich | ||||
Other maps | Fiesta-3 Fiesta x Discovery-3 Discovery-3 Fiesta-3 Fiesta x Totem-3 PI 613988-3 Royal Gala-3 Malling9-3 Robusta5-3 Apple Integrated Map-3 M.27 x M.116-3 | ||||
Reference publication | Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241 | ||||
Remarks | none | ||||
Sequence | |||||
>CH03g07 aattccttttactctctctctctctctctctcacgagaacaaaatcacgcgttgacatcataacaaaactcgatttagaa aaaagaatgtaccatataaataagcattcaaagcaatccgagtgctccggcgaggtgacagtctctctctctctctctct ctctcacataacgaaaacaaaatcacgcattgacataacaacgaaactcgatttggaaaaagaacgtaccatataaataa gcattcgaagcaggccgagcgctccggcgaggtgacagtcagtccactggacaaggaagaggaaaaagttggccgccggg acagtaagacagtctcatctgaagacgcgctccatctttctccctcgggataatccaaggccctggaaaaaacagagagc cnaacaaagcatttgatcaacaagcattccacaatt | |||||
User's notes | |||||
THERE ARE NO USER'S NOTES. | |||||
Allele size and gel image | |||||
Cultivar | Allele size | ||||
Prima Fiesta Discovery Durello di Forlí TN10-8 Florina Nova Easygro Starking |
129 119:123 123:171 119:127 123:127 123:129 127:129 |
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