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CH03g07
Primer features
Name Sequence Length Tm  
CH03g07.F AATAAGCATTCAAAGCAATCCG 22 nt 56.5 °C Primer Stats
CH03g07.R TTTTTCCAAATCGAGTTTCGTT 22 nt 54.7 °C
SSR info
SSR repeat type Perfect
Locus type single locus
Detected loci
Alleles size range 119-171
Number of alleles detected 5
Expected heterozygosity 0.77
PCR annealing temp 60
Sequenced allele size 123
Linkage group(s)
Developed by ETH Zuerich
Other maps Fiesta-3 Fiesta x Discovery-3 Discovery-3 Fiesta-3 Fiesta x Totem-3 PI 613988-3 Royal Gala-3 Malling9-3 Robusta5-3 Apple Integrated Map-3 M.27 x M.116-3
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH03g07 aattccttttactctctctctctctctctctcacgagaacaaaatcacgcgttgacatcataacaaaactcgatttagaa aaaagaatgtaccatataaataagcattcaaagcaatccgagtgctccggcgaggtgacagtctctctctctctctctct ctctcacataacgaaaacaaaatcacgcattgacataacaacgaaactcgatttggaaaaagaacgtaccatataaataa gcattcgaagcaggccgagcgctccggcgaggtgacagtcagtccactggacaaggaagaggaaaaagttggccgccggg acagtaagacagtctcatctgaagacgcgctccatctttctccctcgggataatccaaggccctggaaaaaacagagagc cnaacaaagcatttgatcaacaagcattccacaatt
User's notes
THERE ARE NO USER'S NOTES.
Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
129
119:123
123:171

119:127
123:127
123:129
127:129


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