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CH04d02
Primer features
Name Sequence Length Tm  
CH04d02.F CGTACGCTGCTTCTTTTGCT 20 nt 58.4 °C Primer Stats
CH04d02.R CTATCCACCACCCGTCAACT 20 nt 60.5 °C
SSR info
SSR repeat type Perfect
Locus type single locus
Detected loci
Alleles size range 118-146
Number of alleles detected 3
Expected heterozygosity 0.54
PCR annealing temp 60
Sequenced allele size 144
Linkage group(s)
Developed by ETH Zuerich
Other maps Fiesta x Discovery-12 Discovery-12 Fiesta-12 Fiesta x Totem-12 PI 613988-12 Royal Gala-12 Apple Integrated Map-12 M.27 x M.116-12
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH04d02 aattctaactaatacaaacgncgtacgctgcttcttttgctggtcgatctctctctctctctctctctctctctctctct ctctctgagattcaggtcgggcatcagtgggatgggtcgtggaggacggggtgtgcgggaaccggagttgacgggtggtg gataggatcgcggagggggaggtggcggaggttggtcncatagggtggtanttctttgggttcccttttctttaaatcac tatcattgactttcatcaatt
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
120
118:120
120:146

120
120:146
120:146
120


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