Select a marker

CH04e02
Primer features
Name Sequence Length Tm  
CH04e02.F GGCGATGACTACCAGGAAAA 20 nt 58.4 °C Primer Stats
CH04e02.R ATGTAGCCAAGCCAGCGTAT 20 nt 58.4 °C
SSR info
SSR repeat type Imperfect
Locus type single locus
Detected loci
Alleles size range 143-163
Number of alleles detected 6
Expected heterozygosity 0.72
PCR annealing temp 60
Sequenced allele size 159
Linkage group(s)
Developed by ETH Zuerich
Other maps Discovery-4 Fiesta-4 Fiesta x Totem-4 Royal Gala-4 Apple Integrated Map-4 M.27 x M.116-4
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH04e02 aattgagggagctggtgagggttgcttaaatacaagtgcacatcatttaatgcggcgatgactaccaggaaaacagttat aatgagagagagagagagagaganagagagggacacagagaggatggttaggttattgaaggggaantacatttgggtag gtacacgacagatcatagatggacgatggtggatacgctggcttggctacattcaaagattacagggcattgtttggttg gttggtaccgaaccggtgattttcgtanaaagtttggtccctgaagttgaactggtgattttggtggaaagtttggtccg ttttgttgtctatttccacgttttgttaatt
User's notes
THERE ARE NO USER'S NOTES.
Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
155:163
147:155
155:163

155
147:155
149:155
149:151


admin Send comments to Luca Gianfranceschi