CH04e02
Primer features | |||||
Name | Sequence | Length | Tm | ||
CH04e02.F | GGCGATGACTACCAGGAAAA | 20 nt | 58.4 °C | ||
CH04e02.R | ATGTAGCCAAGCCAGCGTAT | 20 nt | 58.4 °C | ||
SSR info | |||||
SSR repeat type | Imperfect | ||||
Locus type | single locus | ||||
Detected loci | |||||
Alleles size range | 143-163 | ||||
Number of alleles detected | 6 | ||||
Expected heterozygosity | 0.72 | ||||
PCR annealing temp | 60 | ||||
Sequenced allele size | 159 | ||||
Linkage group(s) | |||||
Developed by | ETH Zuerich | ||||
Other maps | Discovery-4 Fiesta-4 Fiesta x Totem-4 Royal Gala-4 Apple Integrated Map-4 M.27 x M.116-4 | ||||
Reference publication | Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241 | ||||
Remarks | none | ||||
Sequence | |||||
>CH04e02 aattgagggagctggtgagggttgcttaaatacaagtgcacatcatttaatgcggcgatgactaccaggaaaacagttat aatgagagagagagagagagaganagagagggacacagagaggatggttaggttattgaaggggaantacatttgggtag gtacacgacagatcatagatggacgatggtggatacgctggcttggctacattcaaagattacagggcattgtttggttg gttggtaccgaaccggtgattttcgtanaaagtttggtccctgaagttgaactggtgattttggtggaaagtttggtccg ttttgttgtctatttccacgttttgttaatt | |||||
User's notes | |||||
THERE ARE NO USER'S NOTES. | |||||
Allele size and gel image | |||||
Cultivar | Allele size | ||||
Prima Fiesta Discovery Durello di Forlí TN10-8 Florina Nova Easygro Starking |
155:163 147:155 155:163 155 147:155 149:155 149:151 |
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