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CH04f04
Primer features
Name Sequence Length Tm  
CH04f04.F GTCGGTCACAACTCAGGACC 20 nt 62.5 °C Primer Stats
CH04f04.R CGACGTTCGATCTTCCTCTC 20 nt 60.5 °C
SSR info
SSR repeat type Perfect
Locus type presumed single locus
Detected loci
Alleles size range 144-166
Number of alleles detected 5
Expected heterozygosity 0.7
PCR annealing temp 60
Sequenced allele size 168
Linkage group(s)
Developed by ETH Zuerich
Other maps Fiesta x Totem-5
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH04f04 aattgcaccagaaatatcgtcggtcacaactcaggacccgcacacgactctgcttacaatgtcccttccagaacgtcgct tgcgaagagagagagagagagagagagagagagagagagagagacgggaggcctggagagagagttgataatgatgacag agggaggagaggaagatcgaacgtcgttgcctcnaaatgggatttgtnaccgtgtcaggatttgttaaaatccactctat tgtggactcaccaactcntcctgtccaaatt
User's notes
Luca Gianfranceschi (luca.gianfranceschi@unimi.it)
Date 13 September 2005 - Time: 15:10
Forward primer sequence was wrong. Please use the new one.

Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
150
146:150
150

150:152
146:166
150:166
166


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