CH04g04
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| CH04g04.F | AGTGGCTGATGAGGATGAGG | 20 nt | 60.5 °C |  |
|
| CH04g04.R | GCTAGTTGCACCAAGTTCACA | 21 nt | 59.4 °C | ||
| SSR info | |||||
| SSR repeat type | Perfect | ||||
| Locus type | single locus | ||||
| Detected loci | |||||
| Alleles size range | 170-186 | ||||
| Number of alleles detected | 5 | ||||
| Expected heterozygosity | 0.73 | ||||
| PCR annealing temp | 60 | ||||
| Sequenced allele size | 183 | ||||
| Linkage group(s) | |||||
| Developed by | ETH Zuerich | ||||
| Other maps | Fiesta x Discovery-12 Discovery-12 Fiesta-12 Fiesta x Totem-12 PI 613988-12 Malling9-12 Apple Integrated Map-12 M.27 x M.116-12 | ||||
| Reference publication | Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241 | ||||
| Remarks | none | ||||
| Sequence | |||||
| >CH04g04 aatttcagtagtggctgatgaggatgaggataataacaagtaggtcaaaaggatatgtgggcaaatgaatgggggatttt attcgttgtaactcagagagagagagagagagagagagagagagagagagagagagagagagagagtatttgaggttaat taatttagaagtgtgaacttggtgcaactagcttcaacttgcaatatgttgagagattcttatctatataaacagatgct ggataaagtgttggatttatcattgtcgtcctcntcttttttaatctttgtgttttaatt | |||||
| User's notes | |||||
| THERE ARE NO USER'S NOTES. | |||||
| Allele size and gel image | |||||
| Cultivar | Allele size |  | |||
|
Prima Fiesta Discovery Durello di Forlí TN10-8 Florina Nova Easygro Starking |
172:180 170:180 176:186 172:180 180:186 172:180 172:186 |
||||
Send comments to Luca Gianfranceschi
