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CH04g09
Primer features
Name Sequence Length Tm  
CH04g09.F TTGTCGCACAAGCCAGTTTA 20 nt 56.4 °C Primer Stats
CH04g09.R GAAGACTCATGGGTGCCATT 20 nt 58.4 °C
SSR info
SSR repeat type Perfect
Locus type multi-locus
Detected loci
Alleles size range 141-177
Number of alleles detected 11
Expected heterozygosity n.d.
PCR annealing temp 60
Sequenced allele size 157
Linkage group(s)
Developed by ETH Zuerich
Other maps Discovery-5 Discovery-10 Fiesta x Totem-10 Fiesta x Totem-15 Malling9-5 Robusta5-5 Apple Integrated Map-5 Apple Integrated Map-10 M.27 x M.116-5
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH04g09 aattgtagctttgatgccaacagtggcctataaagtccattgtcgcacaagccagtttattagttcaagaccagtgttct gctctctctctctctctctctctctctctctctctctctctccgtgtgtttatcttcatctctctcacagtcacacactc ggagagtgctagctgcaatggcacccatgagtcttcctcctggatttagattccacccaacagatgaagagcttgttgct tactatctggatcgaaaaatcaatggtcggaccattgagctagaaatt
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
151:153:169
147:151:151
143:149:177

153:157
141:145:155
145:149
145:155


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