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CH05a02
Primer features
Name Sequence Length Tm  
CH05a02.F GTTGCAAGAGTTGCATGTTAGC 22 nt 60.3 °C Primer Stats
CH05a02.R TTTTGACCCCATAAAACCCAC 21 nt 57.4 °C
SSR info
SSR repeat type Perfect
Locus type multi-locus
Detected loci
Alleles size range 111-135
Number of alleles detected 7
Expected heterozygosity n.d.
PCR annealing temp 60
Sequenced allele size 126
Linkage group(s)
Developed by ETH Zuerich
Other maps Discovery x TN10-8-8 Fiesta-8 Discovery-15 Fiesta-8 Fiesta-15 Fiesta x Totem-8 Fiesta x Totem-15 Apple Integrated Map-8
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH05a02 aattgtaaggtgaggagaaaaagatcggggaccttgcgcttgagattgtagcggtgccattcggacttgtaatggagctt ctgctccgagtcgtccanaaactccttgttgcaagagttgcatgttagccctgacattctctctctctctctctctctct ctctctctctctctgtggtttgggataccacngaagaagaaagaggcggcgggtgggttttatggggtcaaaaaaaacgt tncaacggctcgcantcggtgacaatt
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
115:125:129
111:115:125:135
115:117:135:135

115:125:129
115:117:129
115:129:135
115:117:135


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