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CH05a03
Primer features
Name Sequence Length Tm  
CH05a03.F CGGCTGAGCATGGTTACTTC 20 nt 60.5 °C Primer Stats
CH05a03.R TGATCGTTGTGAAAGCTCCA 20 nt 56.4 °C
SSR info
SSR repeat type Perfect
Locus type presumed single locus
Detected loci
Alleles size range 182-220
Number of alleles detected 6
Expected heterozygosity 0.68
PCR annealing temp 60
Sequenced allele size 188
Linkage group(s)
Developed by ETH Zuerich
Other maps Fiesta x Totem-9
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH05a03 aattgcggctgagcatggttacttcttaaggtgtgcctctctctctctctctctctctctctctcgtcaaaactatctca aatgtcagttaagttatagcttgtgtttttgctaatgatggggaaactgaaagataaatacactgtgcttaaaaaaaaaa acttgaacgttaatggagctttcacaacgatcaatatgctacttctcagatggtcacaaaatccaggaaatgggaaacct gtcttcagggtttttgagttttggctggatgaaaactgttgaaccccgtgatgcaatt
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
220
188:190
188:190

190:218
188:190
190:206
190


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