CH05a04
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| CH05a04.F | GAAGCGAATTTTGCACGAAT | 20 nt | 54.3 °C |  |
|
| CH05a04.R | GCTTTTGTTTCATTGAATCCCC | 22 nt | 58.4 °C | ||
| SSR info | |||||
| SSR repeat type | Compound | ||||
| Locus type | single locus | ||||
| Detected loci | |||||
| Alleles size range | 159-189 | ||||
| Number of alleles detected | 8 | ||||
| Expected heterozygosity | 0.83 | ||||
| PCR annealing temp | 60 | ||||
| Sequenced allele size | 191 | ||||
| Linkage group(s) | |||||
| Developed by | ETH Zuerich | ||||
| Other maps | Discovery-16 Fiesta-16 Fiesta x Totem-16 PI 613988-16 Royal Gala-16 Malling9-16 Apple Integrated Map-16 M.27 x M.116-16 | ||||
| Reference publication | Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241 | ||||
| Remarks | none | ||||
| Sequence | |||||
| >CH05a04 AATCCTGAAGCGaattttgcacgaataaaagctataaagatagtatttttatgtgctaattgtgtgtgtgtgtgagagag agagagagagagagagagagagagagagagagagagagagatgcataggaagttgtcggaaaaaaagagagccttctttc tccgtttttattgtgggggattcaatgaaacaaaagctatagaaagctttatctttatggtttgtgctttgttaatt | |||||
| User's notes | |||||
| THERE ARE NO USER'S NOTES. | |||||
| Allele size and gel image | |||||
| Cultivar | Allele size |  | |||
|
Prima Fiesta Discovery Durello di Forlí TN10-8 Florina Nova Easygro Starking |
163:183 165:189 169:nul 181 189 165:189 159:189 |
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