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CH05c04
Primer features
Name Sequence Length Tm  
CH05c04.F CCTTCGTTATCTTCCTTGCATT 22 nt 58.4 °C Primer Stats
CH05c04.R GAGCTTAAGAATAAGAGAAGGGG 23 nt 61.1 °C
SSR info
SSR repeat type Imperfect
Locus type single locus
Detected loci
Alleles size range 186-258
Number of alleles detected 7
Expected heterozygosity 0.8
PCR annealing temp 60
Sequenced allele size 206
Linkage group(s)
Developed by ETH Zuerich
Other maps Fiesta-13 Fiesta x Totem-13 Malling9-13 Robusta5-13 Apple Integrated Map-13 M.27 x M.116-13
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH05c04 aattcatgatgtttatagcaggccaagagccttcgttatcttccttgcattgatgtttcttctttcttcctccccctcta ctctctatctctctcaccctctatctcactccctttctctctttctctctctcaccctctatctctctctttctctctct ctctctctctctctctctctctctctctctctctctctctctctctcttttgccccttctcttattcttaagctctcata gaactttatatccaagtcagtacctttttcactatcagcacccttttatacatccttgcagttttgtaatt
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
186:210
204:258
186

200:210
186:208
186:200
200:208


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