CH05d04
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| CH05d04.F | ACTTGTGAGCCGTGAGAGGT | 20 nt | 60.5 °C |  |
|
| CH05d04.R | TCCGAAGGTATGCTTCGATT | 20 nt | 56.4 °C | ||
| SSR info | |||||
| SSR repeat type | Perfect | ||||
| Locus type | single locus | ||||
| Detected loci | |||||
| Alleles size range | 174-214 | ||||
| Number of alleles detected | 7 | ||||
| Expected heterozygosity | 0.82 | ||||
| PCR annealing temp | 60 | ||||
| Sequenced allele size | 181 | ||||
| Linkage group(s) | |||||
| Developed by | ETH Zuerich | ||||
| Other maps | Discovery x TN10-8-12 Fiesta x Discovery-12 Discovery-12 Fiesta-12 Fiesta x Totem-12 PI 613988-12 Royal Gala-12 Robusta5-12 Apple Integrated Map-12 M.27 x M.116-12 | ||||
| Reference publication | Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241 | ||||
| Remarks | none | ||||
| Sequence | |||||
| >CH05d04 aattacagagaaagaaaatcaactacgataaatcgaatcgaagcgtacctttggatttgaatctacttgtgagccgtgag aggtggatcgtagatttgaatagctctctctctctctctctctctctctctctctctctctctctctctttaccgctgat taagatgcagaacatatgaaatcgaatgtgattgcagagaaaggaaatcgactacgacagtatcgaatcgaagcatacct tcggatttgtatctgcttgcgagccgcgaaaganatggatcngttggatttgaagacttgaagagctttctccctgtccc tcttccagttagccagatacgaagggttggggggaaanaaaaaaacgctccattgcccgccttaccgccattttcccttt tccgtttcccctcccccaaatt | |||||
| User's notes | |||||
| THERE ARE NO USER'S NOTES. | |||||
| Allele size and gel image | |||||
| Cultivar | Allele size |  | |||
|
Prima Fiesta Discovery Durello di Forlí TN10-8 Florina Nova Easygro Starking |
176:186 176:214 178:182 176 180:182 180 180:182 |
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