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CH05e06
Primer features
Name Sequence Length Tm  
CH05e06.F ACACGCACAGAGACAGAGACAT 22 nt 62.1 °C Primer Stats
CH05e06.R GTTGAATAGCATCCCAAATGGT 22 nt 58.4 °C
SSR info
SSR repeat type Imperfect
Locus type single locus
Detected loci
Alleles size range 125-222
Number of alleles detected 8
Expected heterozygosity 0.85
PCR annealing temp 60
Sequenced allele size 151
Linkage group(s)
Developed by ETH Zuerich
Other maps Discovery-5 Fiesta-5 Fiesta x Totem-5 Royal Gala-5 Robusta5-5 Apple Integrated Map-5
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH05e06 aattttcacacacacgcacagagacagagacatagacacagagagagagagagagagagagagagagagagagagagaga tagagagagagagagagagagagacagagatacagagaatatgtaccggcctaaatgggaaaccaccatttgggatgcta ttcaacttattcttgagatgtgatacttcagctggtgcatctgatagtttcgtgtcatcaccatttacttctgtctcatt caaagtcccagactgaggagccagactaaatggaaactctggccgacctcctttttgtctgccgtttgaccttgagaatt
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
134:216
216:222
125:150

150
140:150
125:146
140:146


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