CH05f06
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| CH05f06.F | TTAGATCCGGTCACTCTCCACT | 22 nt | 62.1 °C |  |
|
| CH05f06.R | TGGAGGAAGACGAAGAAGAAAG | 22 nt | 60.3 °C | ||
| SSR info | |||||
| SSR repeat type | Perfect | ||||
| Locus type | single locus | ||||
| Detected loci | |||||
| Alleles size range | 166-184 | ||||
| Number of alleles detected | 5 | ||||
| Expected heterozygosity | 0.74 | ||||
| PCR annealing temp | 60 | ||||
| Sequenced allele size | 175 | ||||
| Linkage group(s) | |||||
| Developed by | ETH Zuerich | ||||
| Other maps | Discovery-5 Discovery-5 Fiesta-5 Fiesta x Totem-5 Malling9-5 Robusta5-5 Apple Integrated Map-5 M.27 x M.116-5 | ||||
| Reference publication | Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241 | ||||
| Remarks | none | ||||
| Sequence | |||||
| >CH05f06 aattactagttactacctacaaatacaagttactagttattcgttgcttcgttggaactagtgttgacattagatccggt cactctccactgaagtaaagaaactctctctctctctctctctctctctctctctctccacccgcgtgtgacaganaagt gctttgtcatccacgacccctgccttttactttgactccaactgcgtgcttctcaggtacttctttcttcttcgtcttcc tccattatctctctccttctttacaccttttccattttttaatt | |||||
| User's notes | |||||
| THERE ARE NO USER'S NOTES. | |||||
| Allele size and gel image | |||||
| Cultivar | Allele size | ||||
|
Prima Fiesta Discovery Durello di Forlí TN10-8 Florina Nova Easygro Starking |
180:184 166:176 166:184 180:184 176:180 176:184 176:184 |
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