CN444542-SSR
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| CN444542-SSR.F | ATAAGCCAGGCCACCAAATC | 20 nt | 58.4 °C |  |
|
| CN444542-SSR.R | GTTTGCAGTGGATTGATGTTCC | 22 nt | 60.3 °C | ||
| SSR info | |||||
| SSR repeat type | Perfect | ||||
| Locus type | presumed multi-locus | ||||
| Detected loci | |||||
| Alleles size range | 110-156 | ||||
| Number of alleles detected | 8 | ||||
| Expected heterozygosity | n.d. | ||||
| PCR annealing temp | 60 | ||||
| Sequenced allele size | 0 | ||||
| Linkage group(s) | |||||
| Developed by | HiDRAS consortium | ||||
| Other maps | Discovery-9 Fiesta-9 91.136 B6-77-9 Fiesta x Totem-9 PI 613988-9 Royal Gala-9 Apple Integrated Map-9 M.27 x M.116-9 M.27 x M.116-13 | ||||
| Reference publication | Silfverberg-Dilworth E, Matasci C L, Van de Weg W E, Van Kaauwen M P W,Walser M, Kodde L P, Soglio V, Gianfranceschi L, Durel C E, Costa F, Yamamoto T, Koller B, Gessler C, Patocchi A (2006) Microsatellite markers spanning the apple (Malus x domestica Borkh) genome. Tree Genetics & Genomes 2(4): 202-224 | ||||
| Remarks | clean - Possibly the same locus as NZmsCN444542 | ||||
| Sequence | |||||
| >gi|46423806|gb|CN444542.1|CN444542 Mdfw2003g22.x1 Mdfw Malus x domestica cDNA clone Mdfw2003g22 3' similar to TR:Q9SSL1 Q9SSL1 F15H11.6 PROTEIN. ;, mRNA sequence CCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAACTCATAAAAACCGATCACTCCATTTCACAT CTTTATGAACAAAAGAATTGAATAAGCCAGGCCACCAAATCCAACTGTATACATGGAGAGAGAAANANAG AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCAATTGCAATCTACAATCTACAGACCCCTAAAAC TAGGAACATCAATCCACTGCAACTGGAGTTCAACCTCCCCACACTCCACATTTCTGAATCGGAAGATCAT GTTTTGAACGAGTTTGCCGTTGGACCAAATAATGCAGCTCTCTTCAACAAGGCAATTTTCCCTGCTTGGT TGTACTTTTGTAAATACGGTTCCATCTGGTAAACCTGCCAATTGCATCCTCAAGACTTTGATAAATAGTG CAATATCAAACTCTGCGTCCCCCATTTTGTCATCAAAACTAAATGTATCTTTATCGTACACAAAAAGCGT GATCGGAAGATTTGGTTCTGTAACAGAAAGTGTCAATCTTTCATCCCATTCTGGATTCACATTCCTCTTC ACTACAAAAGTTTTCAGTTTCTGCTTGCCCATTTTAACAACAACAAAAGGATCACTGCT | |||||
| User's notes | |||||
| LucaG (luca.gianfranceschi@unimi.it) Date 13 February 2006 - Time: 10:11 Sequence Assembly Library View Sequence Info at PlantGDB | |||||
| Allele size and gel image | |||||
| Cultivar | Allele size |  | |||
|
Fiesta Discovery Gala Florina Nova Easygro TN10-8 Durello di Forli Prima Modial Gala Fuji |
110:136:146 110:128:136:156 110:136:146 110:124:136 110:124:136 110:126:146 110:136 110:124:136 |
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