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CN444794-SSR
Primer features
Name Sequence Length Tm  
CN444794-SSR.F CATGGCAGGTGCTAAACTTG 20 nt 58.4 °C Primer Stats
CN444794-SSR.R GTTTGCAACTCACACAATGCAAC 23 nt 61.1 °C
SSR info
SSR repeat type Perfect
Locus type presumed multi-locus
Detected loci
Alleles size range 230-306
Number of alleles detected 8
Expected heterozygosity n.d.
PCR annealing temp 60
Sequenced allele size 0
Linkage group(s)
Developed by HiDRAS consortium
Other maps Discovery-7 Fiesta-7 Fiesta x Totem-7 Malling9-7 Robusta5-7 Apple Integrated Map-7 M.27 x M.116-7
Reference publication Silfverberg-Dilworth E, Matasci C L, Van de Weg W E, Van Kaauwen M P W,Walser M, Kodde L P, Soglio V, Gianfranceschi L, Durel C E, Costa F, Yamamoto T, Koller B, Gessler C, Patocchi A (2006) Microsatellite markers spanning the apple (Malus x domestica Borkh) genome. Tree Genetics & Genomes 2(4): 202-224
Remarks extra bands
Sequence
>gi|46424058|gb|CN444794.1|CN444794 Mdfw2001i05.y1 Mdfw Malus x domestica cDNA clone Mdfw2001i05 5', mRNA sequence TCTAGCGCTTTTTGTGATGTAGTGTTGTGAAGATTGTGACGGAGCAAATACTTTCGTGGCCCATGGCAGG TGCTAAACTTGATAATTGTGGATAATGGTCCGAATACAACAGATTTGGGTATGAAAATTTGAATATATAT TGGATATGGAAAAGACTGAATATTATTTGGTTATGGCTTTGAATATGGTTATAGGGATTCCCGATTTGTA TCCGTCCCCGAATCCAATCCAAATAATATATATATATATATATTTAGTATTCCCGAATCCATTACCTTGA GAATACAAAAGTTGCATTGTGTGAGTTGCATTTTGTGGGATGTTCAAAGTTTTGAATTAAAATCAGTGAG AATTCAATGGTACTAAACCAACATCATCAATGTTTTAAATTCAATACTTTAGTTTTTATACTCCCCAAGA TCCGTTCATTGAA
User's notes
LucaG (luca.gianfranceschi@unimi.it)
Date 13 February 2006 - Time: 10:18

Sequence
Title: Mdfw2001i05.y1 Mdfw Malus x domestica cDNA clone Mdfw2001i05 5', mRNA sequence.
Type: mRNA
Length: 433 bp
Organism: Malus x domestica
GenBank Accession: CN444794

Assembly
This sequence was assembled into contig

Library
Name: Mdfw
Cultivar: GoldRush
Tissue:Flower

View Sequence Info at PlantGDB
Allele size and gel image
Cultivar Allele size
Fiesta
Discovery
Gala
Florina
Nova Easygro
TN10-8
Durello di Forli
Prima
Modial Gala
Fuji
 		260:306
230:298
254:270
270
260
230

256:272

256

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