CN445599-SSR
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| CN445599-SSR.F | TCAAATGGGTTCGATCTTCAC | 21 nt | 57.4 °C |  |
|
| CN445599-SSR.R | GTTTGCCTGGCTGTAACTGTTTGG | 24 nt | 65.2 °C | ||
| SSR info | |||||
| SSR repeat type | Perfect | ||||
| Locus type | presumed multi-locus | ||||
| Detected loci | |||||
| Alleles size range | 130-176 | ||||
| Number of alleles detected | 10 | ||||
| Expected heterozygosity | n.d. | ||||
| PCR annealing temp | 60 | ||||
| Sequenced allele size | 0 | ||||
| Linkage group(s) | |||||
| Developed by | HiDRAS consortium | ||||
| Other maps | Discovery-5 Fiesta-5 Robusta5-5 | ||||
| Reference publication | Silfverberg-Dilworth E, Matasci C L, Van de Weg W E, Van Kaauwen M P W,Walser M, Kodde L P, Soglio V, Gianfranceschi L, Durel C E, Costa F, Yamamoto T, Koller B, Gessler C, Patocchi A (2006) Microsatellite markers spanning the apple (Malus x domestica Borkh) genome. Tree Genetics & Genomes 2(4): 202-224 | ||||
| Remarks | extra bands | ||||
| Sequence | |||||
| >gi|46424863|gb|CN445599.1|CN445599 Mdfw2003f11.y1 Mdfw Malus x domestica cDNA clone Mdfw2003f11 5' similar to TR:O81062 O81062 T18E12.21 PROTEIN. ;, mRNA sequence TTTTCTCAAAATCTCTTCTCTCCGATCCGTACGGAAGGAGACCGTTTGAAAATCTTCTGCATCGCGGCCC ACTGGGTTTTGAGGAGGAGGCCCAATTCAGTTCGATTTGATCGGAATTGTTGTTCGAAGCGGTTTCTTTT CGAGTGATTACATTAACGATGAAGAACGTTGAGCGTATTGCCAATATCGCCTTAGCAGGTTTAACGTTAG CACCACTTGTTGTAAAGGTAGATCCAAACCTAAATGTAGTTTTGACTGCATGTCTCACAGTTTTCGTGGG TTGCTACCGGTCTGTGAAGCCAACTCCACCTTCAGAGACGATGTCCAATGAACATGCCATGCGTTTCCCG TTTGTCGGGAGTGCAATGCTGTTATCACTATTTTTACTTTTTAAGTTCTTATCCAAAAACTTGGTTAATG CTGTCTTGACGTGCTATTTCTTTGTTCATGGGATAATTGCTCTT | |||||
| User's notes | |||||
| LucaG (luca.gianfranceschi@unimi.it) Date 13 February 2006 - Time: 10:22 Sequence Assembly Library View Sequence Info at PlantGDB | |||||
| Allele size and gel image | |||||
| Cultivar | Allele size |  | |||
|
Fiesta Discovery Gala Florina Nova Easygro TN10-8 Durello di Forli Prima Modial Gala Fuji |
131:154:164 130:132:152:176 130:132:146:154 130:146:176 131:136 131:132:150:154 131:136:154 130:131:146 |
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