CN496002-SSR
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| CN496002-SSR.F | TCAGAATCTCAAGCAAGATCCTC | 23 nt | 61.1 °C |  |
|
| CN496002-SSR.R | GTTTGATTGATCGTGGCGATATG | 23 nt | 61.1 °C | ||
| SSR info | |||||
| SSR repeat type | Perfect | ||||
| Locus type | single locus | ||||
| Detected loci | |||||
| Alleles size range | 243-261 | ||||
| Number of alleles detected | 4 | ||||
| Expected heterozygosity | n.d. | ||||
| PCR annealing temp | 60 | ||||
| Sequenced allele size | 0 | ||||
| Linkage group(s) | |||||
| Developed by | HiDRAS consortium | ||||
| Other maps | Discovery-5 Fiesta-5 | ||||
| Reference publication | Silfverberg-Dilworth E, Matasci C L, Van de Weg W E, Van Kaauwen M P W,Walser M, Kodde L P, Soglio V, Gianfranceschi L, Durel C E, Costa F, Yamamoto T, Koller B, Gessler C, Patocchi A (2006) Microsatellite markers spanning the apple (Malus x domestica Borkh) genome. Tree Genetics & Genomes 2(4): 202-224 | ||||
| Remarks | clean | ||||
| Sequence | |||||
| >gi|46597951|gb|CN496002.1|CN496002 Mdfw2021d09.y1 Mdfw Malus x domestica cDNA clone Mdfw2021d09 5' similar to TR:O23131 O23131 HYPOTHETICAL 37.1 KD PROTEIN. ;, mRNA sequence AAAATCACCATTCAGAATCTCAAGCAAGATCCTCTTCTTCTTCTTGTACCGCCGCCGCCACCACTAACAG CACCAGCAGCAGCTAGGCTAGAGCTAGATCTATTGTTAGTGTAGTGCATACCAATTATTAGTAGCTAGAT ATATATATATATATATATATATAGGACTCAATTCGTAATTAGTAACATCAACACCACCGCACAGGATTTC TAAGAGATCGATCGAGAGCTGATCATATAATCATATCGCCACGATCAATCAAGATTTGAAGATGACTCCA GCTAATCTGGCAAGGCAATTTGGCGACACAACTTACACCAAAGAGTTTGTTGGAGGGTTGGCATGAGAAA CCCAGAAGGAAACCATGAAGAATAACTTTGAA | |||||
| User's notes | |||||
| LucaG (luca.gianfranceschi@unimi.it) Date 13 February 2006 - Time: 14:14 Sequence Assembly Library View Sequence Info at PlantGDB | |||||
| Allele size and gel image | |||||
| Cultivar | Allele size |  | |||
|
Fiesta Discovery Gala Florina Nova Easygro TN10-8 Durello di Forli Prima Modial Gala Fuji |
243:257 243:259 243:259 257:261 nd 243:261 243:257 243:257 |
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