COL
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| COL.F | AGGAGAAAGGCGTTTACCTG | 20 nt | 58.4 °C |  |
|
| COL.R | GACTCATTCTTCGTCGTCACTG | 22 nt | 62.1 °C | ||
| SSR info | |||||
| SSR repeat type | Perfect | ||||
| Locus type | single locus | ||||
| Detected loci | |||||
| Alleles size range | 220-240 | ||||
| Number of alleles detected | 5 | ||||
| Expected heterozygosity | 0.76 | ||||
| PCR annealing temp | 60 | ||||
| Sequenced allele size | 237 | ||||
| Linkage group(s) | |||||
| Developed by | ETH Zuerich | ||||
| Other maps | Discovery x TN10-8-10 Braeburn-15 Telamon-15 Spur-Fuji x Telamon-10 Discovery-10 Fiesta-10 Fiesta-10 Mondial Gala-10 | ||||
| Reference publication | Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241 | ||||
| Remarks | none | ||||
| Sequence | |||||
| >gi|1236655|gb|U50187.1|MDU50187 Malus domestica simple sequence repeat linked to the columnar locus (Co) CAATCGCCGTTAAGCAAAGTAATAATCGGTAATTAACACAGATTTCACAGTTTCANGCGTNGATTTCTTG TTGCATGCTATATAAATCACATAATCTAAATCTAATCGCATCANCTTAAGAAAATCAGTTCAGAAATCTC AAATGTCCAATTATTTACTACTAAAAATCGCTTCGAAAACCTCAAGAGATCATCAAAAATTCCAGAACGA TAATCCCAAATTAATCATGTAAAAAAAGGAGAAAGGCGTTTACCTGAGAGAGCTCCAAACGCAGCGCTTG AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGCAAATGGACAAGTAGAAGGAGGGAAGGGGAAGGCG ACGCCCTGAAAAATCTAACACACAATTGTGACTTAGCCGCCGCGTTATCGTATTTGACGTGGCGCACGCA CAGTCAGCGTCCCGGCACAAACTTCAATGACCAGTGACGACGAAGAATGAGTCAATCGTGCGACGCCATG TGTGACGTGTTTGTTGATATTTCTTGTTCCGCATGTGAGCTGCGTGACGTGCTTGTTTGATCCGCTTCGT TTCAACTCTTCTGACATTTGATATTTTTTTAGGGTATGATATCCATACATCCTATATTATTTCTCACACA TTTTTTTTTTAATTTTTTAACTGTCGGATTGGATGAATTAAAGAATATCAACGAACAGAAATTATCAAAA TGTATCTGAGAAGTAATATAGAATGCGTGGATAGTACACTCCTTTTTCTCTTTTGCTCATTTTTCTTTCT AACTGGGCCTGGTTTANTCTCTTATTGGCCCATCATGGAGAAAACCTTGGGTCGGCANTGTNGGGGCCAT CCGGAAAGCCACATAAATGTCCCCCGCACACAAAAATTCCTTATTTCTATGTATTTTGGACTCACCGCTT CTGTTAGTTTGGCAACCTTATATGGGTGTCCAGACTGAAGAAATCTCCTTACGCAGGCGCCCGGAGTCCC GACACATGACTTTTCTTGTACGGACTTTCCCAACCACGGCGATTG | |||||
| User's notes | |||||
| Luca Gianfranceschi (luca.gianfranceschi@unimi.it) Date 13 September 2005 - Time: 14:54 Malus domestica simple sequence repeat linked to the columnar locus (Co). PCR primers developed from public sequence. See Hemmat et al. A DNA marker for columnar growth habit in apple contains a simple sequence repeat. J. Am. Soc. Hort. Sci. 122 (3), 347-349 (1997.) LucaG (luca.gianfranceschi@unimi.it) Date 13 February 2006 - Time: 15:40 ATTENTION! This markers is the same as U50187-SSR | |||||
| Allele size and gel image | |||||
| Cultivar | Allele size |  | |||
|
Prima Fiesta Discovery Durello di Forlí TN10-8 Florina Nova Easygro Starking |
222:232 230:232 230:240 220:230 222:232 222:240 222:232 |
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