EMPc117
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| EMPc117.F | GTTCTATCTACCAAGCCACGCT | 22 nt | 62.1 °C |  |
|
| EMPc117.R | CGTTTGTGTGTTTTACGTGTTG | 22 nt | 58.4 °C | ||
| SSR info | |||||
| SSR repeat type | unknown | ||||
| Locus type | |||||
| Detected loci | |||||
| Alleles size range | 76-119 | ||||
| Number of alleles detected | 17 | ||||
| Expected heterozygosity | 0.91 | ||||
| PCR annealing temp | 0 | ||||
| Sequenced allele size | 0 | ||||
| Linkage group(s) | |||||
| Developed by | East Malling Research (UK) | ||||
| Other maps | Fiesta x Totem-7 Robusta5-7 M.27 x M.116-7 | ||||
| Reference publication | Fernandez-Fernandez, F; Harvey, NG; James, CM (2006) Isolation and characterization of polymorphic microsatellite markers from European pear (Pyrus communis). Molecular Ecology Notes 6(): 1039?1041 | ||||
| Remarks | polymorphic amplification in Malus | ||||
| Sequence | |||||
| >gi|118402608|emb|AM182398.1| Pyrus communis satellite DNA, clone EMPc117 GGGCCGACGTCGCATGCTCCCGGNCNNCATGGCGGCCGCGGGAATTCGATTCTCTNGCTTACGCGTGGAC TACTTATGGGTGATGAAACTAATAAGTGTCACAGTTAACAGAATCACAGACAAAGTAAACAACAACATCA ACATCCTTCCCAGCCCCAAAGGAGATCAATAATTATGTCATATGTTCTATCTACCAAGCCACGCTGAAAG TAAAATTTAAGTCTCTCTCTCTCCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTACAATACACAAC ACGTAAAACACACAAACGAAAGACCTAGAAAATAATTAAACAAGACTTTTAGTCGTGGAAAATATTCACA TGCATTGAGATGGTGTTTTCATGGGACTGAATCCAACACAGGGTGACTGGGCGATCGAAGGGGTTTGGTG ATGTGTTGTTGAACAACACAGCTAAACGATCTTGCGAGATGCGATGACGTGGTGAATATCTCTCTTAGAT TTTGNTGATGGGGT | |||||
| User's notes | |||||
| THERE ARE NO USER'S NOTES. | |||||
| Allele size and gel image | |||||
| Cultivar | Allele size | ||||
|
Not available |
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