Select a marker

Hi01c04
Primer features
Name Sequence Length Tm  
Hi01c04.F GCTGCCGTTGACGTTAGAG 19 nt 59.5 °C Primer Stats
Hi01c04.R GTTTGTAGAAGTGGCGTTTGAGG 23 nt 62.9 °C
SSR info
SSR repeat type Imperfect
Locus type single locus
Detected loci
Alleles size range 214-232
Number of alleles detected 4
Expected heterozygosity n.d.
PCR annealing temp 60
Sequenced allele size 0
Linkage group(s)
Developed by HiDRAS consortium
Other maps Discovery-5 Fiesta-5 Malling9-5 Robusta5-5
Reference publication Silfverberg-Dilworth E, Matasci C L, Van de Weg W E, Van Kaauwen M P W,Walser M, Kodde L P, Soglio V, Gianfranceschi L, Durel C E, Costa F, Yamamoto T, Koller B, Gessler C, Patocchi A (2006) Microsatellite markers spanning the apple (Malus x domestica Borkh) genome. Tree Genetics & Genomes 2(4): 202-224
Remarks clean, compl. band(s)
Sequence
>Hi01C04 AACAATGCGCGGCTGCCGTTGACGTTAGAGTCTACGCTCCTCTCTCTCCTCTCTCTCCTC TCTCTCTCTCTCTACCGTTTATAAATCCCGTCCTCAAACGCCACTTCTACATCACCGCCC CTCCCTCTGAAATCCTCTCCCTCTCTACGCTCCTCTCTCTCCTCTCTCTTCTCTCTCTTC TCTCTCTCTACCGTTTATAAATCCCCTGTCCTCAAACGCCACTTCTACATCACCACCCCC CCCTCTGAAAATCCTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTGTGAAAAAGCTCTCT CTCTCTCTCTCTGTGAAAAAGTTAAACTTTAATTGCAATATGCAATCACAAACTATCCCA TATGGAACATGGATTCCGGGCACCCGAGCCGGCCACGCGACGCCGTCCTCACGCGACGCC TCACGTGAGCAACACCTAGCGGCGGCCAGCGTACAAAAAATGGTGTCGGAGAATGCTGTC ACGGTTGTCGGACGACGTGGCTGCTGCATGTGCCACGTCGTCAAGCGGCTGCTCCTCGGT CACGGGGTCAACCCTACGGTTTTCGGCGATGAGCCAGATCTGAAACAAGGGCGAATTCTG CAGATATCCATCACACTGGCGGCCGCTCGAGCATGCATCTAGAGGGCCCAATTCGC
User's notes
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Allele size and gel image
Cultivar Allele size
Fiesta
Discovery
Gala
Florina
Nova Easygro
TN10-8
Durello di Forli
Prima
Modial Gala
Fuji
218:222
222:232
218
218
214:232
214:218

218

nd


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