Hi01d05
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| Hi01d05.F | GGTATCCTCTTCATCGCCTG | 20 nt | 60.5 °C |  |
|
| Hi01d05.R | TTAGATTGACGTTCCGACCC | 20 nt | 58.4 °C | ||
| SSR info | |||||
| SSR repeat type | Imperfect | ||||
| Locus type | presumed multi-locus | ||||
| Detected loci | |||||
| Alleles size range | 210->330 | ||||
| Number of alleles detected | 16 | ||||
| Expected heterozygosity | n.d. | ||||
| PCR annealing temp | 60 | ||||
| Sequenced allele size | 0 | ||||
| Linkage group(s) | |||||
| Developed by | HiDRAS consortium | ||||
| Other maps | Fiesta-7 M.27 x M.116-6 M.27 x M.116-7 | ||||
| Reference publication | Silfverberg-Dilworth E, Matasci C L, Van de Weg W E, Van Kaauwen M P W,Walser M, Kodde L P, Soglio V, Gianfranceschi L, Durel C E, Costa F, Yamamoto T, Koller B, Gessler C, Patocchi A (2006) Microsatellite markers spanning the apple (Malus x domestica Borkh) genome. Tree Genetics & Genomes 2(4): 202-224 | ||||
| Remarks | extra bands | ||||
| Sequence | |||||
| >Hi01D05 TTTTTGGGCCNCACGTCGCATGCTTCCGGCCGCCATGGCGGCCGCGGGAATTCGATTGTT TCAGATCTGGCTCATCGCCGAAACAATGGTTTGAGAAAAGGAAAAGAGAGACGGACCAGA AGGTTTGGGGTGTCTCTGAGGAAACTTGATGCGAGGTATCCTCTTCATCGCCTGGGCTCC ACCCATCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCcTcTcTCTCTTCT GTAGTACCAGTTCCTCCGGAGACGACGGTTGAAAAAGAAGAGCTCAAATCTTCCACCAGC GATTTGTTTGGCTACTCGGGGATGAACACAAGTTGCTACAAAGGTCAGTTAGTTCAAGGG TGAGGGTTGCCGGGTCGGAACGTCAATCTAAATCGCGATCCACCCTTTTCATAAACTGGG TTGGGCTTCGGGTTCTATCGGGTCGTAAATAGGAAGTTCGTAACGGAATCAAGTTCAACT TTGCTACACGATGGATGAGTAGTTTCTCTTCAAAACACTGTGG | |||||
| User's notes | |||||
| THERE ARE NO USER'S NOTES. | |||||
| Allele size and gel image | |||||
| Cultivar | Allele size |  | |||
|
Fiesta Discovery Gala Florina Nova Easygro TN10-8 Durello di Forli Prima Modial Gala Fuji |
210:242:320:>330 a 210:222:244:300:320:>330 b 212:222:242:300:320 210:222:>330 a/b 212:226:242:>330 a/b 210>330 b 210:222:328 210:242:322:>330 a/b |
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