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Hi03a06
Primer features
Name Sequence Length Tm  
Hi03a06.F TGGTGAGAGAAGGTGACAGG 20 nt 60.5 °C Primer Stats
Hi03a06.R GTTTAAGGCCGGGATTATTAGTCG 24 nt 63.5 °C
SSR info
SSR repeat type Imperfect
Locus type presumed multi-locus
Detected loci
Alleles size range 158-197
Number of alleles detected 5
Expected heterozygosity n.d.
PCR annealing temp 60
Sequenced allele size 0
Linkage group(s)
Developed by HiDRAS consortium
Other maps Discovery-15 Fiesta-15 Apple Integrated Map-13
Reference publication Silfverberg-Dilworth E, Matasci C L, Van de Weg W E, Van Kaauwen M P W,Walser M, Kodde L P, Soglio V, Gianfranceschi L, Durel C E, Costa F, Yamamoto T, Koller B, Gessler C, Patocchi A (2006) Microsatellite markers spanning the apple (Malus x domestica Borkh) genome. Tree Genetics & Genomes 2(4): 202-224
Remarks dirty
Sequence
>Hi03A06 CGACGGGGAGGACGACGACAGCGACGGCGACGGGGAGGACGATGATGGTGAGAGAAGGTG ACAGGGGGGGAGGACGACGACGCCTAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA GAGAGAAGGCCNAGAGAAGGCGAGAGAGAGAGGGCCGACTAATAATCCCGGCCTTTTGGT TGGTTTTATTAGTCCGGTGTATACCGTGCTAAATAAGCACACCGGTNTNATTTAATNCGG CTGCTTATTTAGTACGAGTGAACGCCAAACACCTGCGTCCGTATAATTAGTCCTATCCGA TCCTTCTATCCGATCTG
User's notes
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Allele size and gel image
Cultivar Allele size
Fiesta
Discovery
Gala
Florina
Nova Easygro
TN10-8
Durello di Forli
Prima
Modial Gala
Fuji
158:160:178:197
158:197
158:197
160:178:197
160:178:197
158:160:197

160:178:183:197

nd


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