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Hi03d06
Primer features
Name Sequence Length Tm  
Hi03d06.F TCATGGATCATTTCGGCTAA 20 nt 54.3 °C Primer Stats
Hi03d06.R GTTTGCCAATTTTATCCAGGTTGC 24 nt 61.8 °C
SSR info
SSR repeat type Perfect
Locus type single locus
Detected loci
Alleles size range 115-169
Number of alleles detected 8
Expected heterozygosity n.d.
PCR annealing temp 60
Sequenced allele size 0
Linkage group(s)
Developed by HiDRAS consortium
Other maps Discovery-3 Fiesta x Totem-3 PI 613988-3 Malling9-3 Robusta5-3 Apple Integrated Map-3 M.27 x M.116-3
Reference publication Silfverberg-Dilworth E, Matasci C L, Van de Weg W E, Van Kaauwen M P W,Walser M, Kodde L P, Soglio V, Gianfranceschi L, Durel C E, Costa F, Yamamoto T, Koller B, Gessler C, Patocchi A (2006) Microsatellite markers spanning the apple (Malus x domestica Borkh) genome. Tree Genetics & Genomes 2(4): 202-224
Remarks clean
Sequence
>Hi03D06 CGATTTCGTTGAGTATTTAAGAAGTTATGAATGTTTAAAATTTACCCAGT TTCCGGCGAGTTTTCCAATTTTCCCGTCCATCAGTCACCTTTCAGGCTAT TTTCCGGCTATCTACCGGCAACCATTGGGCTTCCAAATAGGTATAATCTT GTTCTACACTTTCCTATCTTCATTTTGATATATTATGGGTCAAATTTGGT TAAGAAACGATAGAGTTACAAAGTTTTGAAAATTGCCCAAACTTTCAGCC AAGAGCTCCGGGACACTTAACGGAGTTTTTAACGGAATGACCAAAATAAT AGATGGTGGACAATCTCAGAGACCATTTCTATTGATTTTCAATCTCAGGG ACCAAAGTAATGTGTTTTGCAAATCTCATGGATCATTTCGGCTAAAAAGC CATATACAAATGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG TCAAATGTAGGCTAGAAAAATTGATAGCAACCTGGATAAAATTGGCAGAC CATCTGTAT
User's notes
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Allele size and gel image
Cultivar Allele size
Fiesta
Discovery
Gala
Florina
Nova Easygro
TN10-8
Durello di Forli
Prima
Modial Gala
Fuji
117
143:169
117
133:145
145
117:147

117

nd


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