Hi04a08
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| Hi04a08.F | TTGAAGGAGTTTCCGGTTTG | 20 nt | 56.4 °C |  |
|
| Hi04a08.R | GTTTCACTCTGTGCTGGATTATGC | 24 nt | 63.5 °C | ||
| SSR info | |||||
| SSR repeat type | Perfect | ||||
| Locus type | single locus | ||||
| Detected loci | |||||
| Alleles size range | 211-250 | ||||
| Number of alleles detected | 7 | ||||
| Expected heterozygosity | n.d. | ||||
| PCR annealing temp | 60 | ||||
| Sequenced allele size | 0 | ||||
| Linkage group(s) | |||||
| Developed by | HiDRAS consortium | ||||
| Other maps | Discovery-5 Fiesta-5 PI 613988-5 Royal Gala-5 Malling9-5 Robusta5-5 Apple Integrated Map-5 | ||||
| Reference publication | Silfverberg-Dilworth E, Matasci C L, Van de Weg W E, Van Kaauwen M P W,Walser M, Kodde L P, Soglio V, Gianfranceschi L, Durel C E, Costa F, Yamamoto T, Koller B, Gessler C, Patocchi A (2006) Microsatellite markers spanning the apple (Malus x domestica Borkh) genome. Tree Genetics & Genomes 2(4): 202-224 | ||||
| Remarks | clean | ||||
| Sequence | |||||
| >Hi04A08 ACTCTCGCTCCCGGAGCTCCTCAACCAGTCGGAGCAGCTCCGTCACGTCCGGCGGCTTAG GCTGCACCTGCGCGGAGGTGCGTGGGAAGTATACACCAAAGGAGCGCGAGAACACCGCCC CCTTCTGGGGTGTCTTCCCAGAGCTCAACGAAGGCGACGGAGACTTTGAAGGAGTTTCCG GTTTGGGATTCGCCGGCGATTTCTGTAGCCCCATTGCCACCCTCACCTTCCCAGCAACCA TTGCAAAACCCCCTCTCCCTTTCACTCAACACCCTGTTTCTCTCACTCTCTCTCTCTCTC TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTAAAAGTGTGATGTTCCT CTCTCAAGGATTTGGGGTGTGTGATTAGCTTGCATAATCCAGCACAGAGTGAAGGGGTTA C | |||||
| User's notes | |||||
| THERE ARE NO USER'S NOTES. | |||||
| Allele size and gel image | |||||
| Cultivar | Allele size |  | |||
|
Fiesta Discovery Gala Florina Nova Easygro TN10-8 Durello di Forli Prima Modial Gala Fuji |
226:246 216:250 216:226 216:218 212:216 212:216 211:216 nd |
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