Hi04d02
Primer features | |||||
Name | Sequence | Length | Tm | ||
Hi04d02.F | TTCGTGGCTGAGAAAGGAGT | 20 nt | 58.4 °C | ||
Hi04d02.R | GTTTGTACGGTGCATTGTGAAAG | 23 nt | 61.1 °C | ||
SSR info | |||||
SSR repeat type | Perfect | ||||
Locus type | presumed multi-locus | ||||
Detected loci | |||||
Alleles size range | 176-238 | ||||
Number of alleles detected | 14 | ||||
Expected heterozygosity | n.d. | ||||
PCR annealing temp | 60 | ||||
Sequenced allele size | 0 | ||||
Linkage group(s) | |||||
Developed by | HiDRAS consortium | ||||
Other maps | Discovery-5 Fiesta-5 Royal Gala-5 Apple Integrated Map-5 | ||||
Reference publication | Silfverberg-Dilworth E, Matasci C L, Van de Weg W E, Van Kaauwen M P W,Walser M, Kodde L P, Soglio V, Gianfranceschi L, Durel C E, Costa F, Yamamoto T, Koller B, Gessler C, Patocchi A (2006) Microsatellite markers spanning the apple (Malus x domestica Borkh) genome. Tree Genetics & Genomes 2(4): 202-224 | ||||
Remarks | extra bands | ||||
Sequence | |||||
>Hi04D02 ATATCCTTTTGTACGGTGCATTGTGAAAGTTAATGAGAATCATTCTTCACATATTCATTC TATCTCTACAAATTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCAATCTCT TGATTACTGCAAGATATTAGACTTCTACACAAACCTCCAGAGGCTGGAACCAGAATCACC TGCTTCTACGTATCTCCCATGTACTCCTTTCTCAGCCACGAAGCTCCCCGTTGCGGCATT CCACCCCAGAACACATCACGTATCTCATGAACCTTNCCATTCGTGCCAAAATCTCAGAAA AGCTCAAAACTTTATGCATAAGCAAGCAAGAAAAAAGCAAGGTTCTTTAACTTTCCGA | |||||
User's notes | |||||
THERE ARE NO USER'S NOTES. | |||||
Allele size and gel image | |||||
Cultivar | Allele size | ||||
Fiesta Discovery Gala Florina Nova Easygro TN10-8 Durello di Forli Prima Modial Gala Fuji |
208:222 180:194:238 176:222 176:182:204 182:190:204 190:198:222 190:204:214 186:204:218:222 |
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