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Hi04d02
Primer features
Name Sequence Length Tm  
Hi04d02.F TTCGTGGCTGAGAAAGGAGT 20 nt 58.4 °C Primer Stats
Hi04d02.R GTTTGTACGGTGCATTGTGAAAG 23 nt 61.1 °C
SSR info
SSR repeat type Perfect
Locus type presumed multi-locus
Detected loci
Alleles size range 176-238
Number of alleles detected 14
Expected heterozygosity n.d.
PCR annealing temp 60
Sequenced allele size 0
Linkage group(s)
Developed by HiDRAS consortium
Other maps Discovery-5 Fiesta-5 Royal Gala-5 Apple Integrated Map-5
Reference publication Silfverberg-Dilworth E, Matasci C L, Van de Weg W E, Van Kaauwen M P W,Walser M, Kodde L P, Soglio V, Gianfranceschi L, Durel C E, Costa F, Yamamoto T, Koller B, Gessler C, Patocchi A (2006) Microsatellite markers spanning the apple (Malus x domestica Borkh) genome. Tree Genetics & Genomes 2(4): 202-224
Remarks extra bands
Sequence
>Hi04D02 ATATCCTTTTGTACGGTGCATTGTGAAAGTTAATGAGAATCATTCTTCACATATTCATTC TATCTCTACAAATTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCAATCTCT TGATTACTGCAAGATATTAGACTTCTACACAAACCTCCAGAGGCTGGAACCAGAATCACC TGCTTCTACGTATCTCCCATGTACTCCTTTCTCAGCCACGAAGCTCCCCGTTGCGGCATT CCACCCCAGAACACATCACGTATCTCATGAACCTTNCCATTCGTGCCAAAATCTCAGAAA AGCTCAAAACTTTATGCATAAGCAAGCAAGAAAAAAGCAAGGTTCTTTAACTTTCCGA
User's notes
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Allele size and gel image
Cultivar Allele size
Fiesta
Discovery
Gala
Florina
Nova Easygro
TN10-8
Durello di Forli
Prima
Modial Gala
Fuji
208:222
180:194:238
176:222
176:182:204
182:190:204
190:198:222

190:204:214

186:204:218:222


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