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Hi05b09
Primer features
Name Sequence Length Tm  
Hi05b09.F AAACCCAACCCAAAGAGTGG 20 nt 58.4 °C Primer Stats
Hi05b09.R GTTTCTAACGTGCGCCTAACGTG 23 nt 64.6 °C
SSR info
SSR repeat type Perfect
Locus type single locus
Detected loci
Alleles size range 136-144
Number of alleles detected 3
Expected heterozygosity n.d.
PCR annealing temp 60
Sequenced allele size 0
Linkage group(s)
Developed by HiDRAS consortium
Other maps Fiesta-7 Malling9-7 Robusta5-7 M.27 x M.116-7
Reference publication Silfverberg-Dilworth E, Matasci C L, Van de Weg W E, Van Kaauwen M P W,Walser M, Kodde L P, Soglio V, Gianfranceschi L, Durel C E, Costa F, Yamamoto T, Koller B, Gessler C, Patocchi A (2006) Microsatellite markers spanning the apple (Malus x domestica Borkh) genome. Tree Genetics & Genomes 2(4): 202-224
Remarks clean
Sequence
>Hi05B09 CGAAATTGAGAAGAAAACAGTTGGATTGGGAAATAGGGATCTATCTATATATCCAGGAAG TCTCTTTCCAACTGCTTCAGAGCGCAGAGAGAGAGAGAGAGGAGAGAGAAAATCAAATTG TCGTAATTGTAATGTAATTATGTTAAGCAGCGGAAGGGGATATAATATGAGACCTGAATA TTTTTCTGTTTTAATTTTCTGCGAAAGCTTTTAGAGAGAGAAAGAGCGAGGAGAGAGAGA GGGAGCGGGGAGTTAGAGAGAAAGAGAGAGATGGGGGCCGGCGTTATCTTTGCAAAGTGT TATATTGTATAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGGA
User's notes
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Allele size and gel image
Cultivar Allele size
Fiesta
Discovery
Gala
Florina
Nova Easygro
TN10-8
Durello di Forli
Prima
Modial Gala
Fuji
140:144
136
136:140
136
136:144
136:140

140:144

nd


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