Hi07b02
Primer features | |||||
Name | Sequence | Length | Tm | ||
Hi07b02.F | ATTTGGGGTTTCAACAATGG | 20 nt | 54.3 °C | ||
Hi07b02.R | GTTTCGGACATCAAACAAATGTGC | 24 nt | 61.8 °C | ||
SSR info | |||||
SSR repeat type | Imperfect | ||||
Locus type | presumed multi-locus | ||||
Detected loci | |||||
Alleles size range | 212-218 | ||||
Number of alleles detected | 4 | ||||
Expected heterozygosity | n.d. | ||||
PCR annealing temp | 60 | ||||
Sequenced allele size | 0 | ||||
Linkage group(s) | |||||
Developed by | HiDRAS consortium | ||||
Other maps | Fiesta-4 Robusta5-4 Apple Integrated Map-4 M.27 x M.116-4 M.27 x M.116-13 | ||||
Reference publication | Silfverberg-Dilworth E, Matasci C L, Van de Weg W E, Van Kaauwen M P W,Walser M, Kodde L P, Soglio V, Gianfranceschi L, Durel C E, Costa F, Yamamoto T, Koller B, Gessler C, Patocchi A (2006) Microsatellite markers spanning the apple (Malus x domestica Borkh) genome. Tree Genetics & Genomes 2(4): 202-224 | ||||
Remarks | clean | ||||
Sequence | |||||
>Hi07b02 GCCGCCAGTGTGATGGATATCTGCNGAATTCGCCCTTGTTTCAGATCTGGCTCANCGCAT TTTAAAATTTGGGGTTTCAACAATGGGAGTACTCTGTGCGTGTGTGTGTGTGTGTGTGTC TGTGCGTGTGTGTGTGTGTGTTTGTGTACTCTGTGTGTGTGTGTGTAATTTCTCCGTGTG TGTGTGTGTTTATGTGTACTCTGTGTGTGTGTGTATTTAGTTGGTGTGTCTCTGTGTGTG TGTGTGTATTCTGCACATTTGTTTGATGTCCGTGTGTGTGTGTATTCTGCACATTTGAAA GGATAGTGTGTGTATGTGTGTATTCTGCACATTTGTCTGATGTCCGTGTGTGTGTGTGTG TGTGTATTCTGCACATTTGTTTTATGTCTGTGTGTGTGTGCATGCGTGTGTGTGTATTCT GCACATTTGAAAGGATAATGTGTGTGTGTGTGTTCTTCATCG | |||||
User's notes | |||||
THERE ARE NO USER'S NOTES. | |||||
Allele size and gel image | |||||
Cultivar | Allele size | ||||
Fiesta Discovery Gala Florina Nova Easygro TN10-8 Durello di Forli Prima Modial Gala Fuji |
214:218 214 216 212:216 214:216 nd nd 216 |
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