Hi22f12
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| Hi22f12.F | GGCCTCACCCAGTCTACATT | 20 nt | 60.5 °C |  |
|
| Hi22f12.R | GTTTGGTGTGATGGGGTACTTTGC | 24 nt | 65.2 °C | ||
| SSR info | |||||
| SSR repeat type | Imperfect | ||||
| Locus type | single locus | ||||
| Detected loci | |||||
| Alleles size range | 205-217 | ||||
| Number of alleles detected | 4 | ||||
| Expected heterozygosity | n.d. | ||||
| PCR annealing temp | 60 | ||||
| Sequenced allele size | 0 | ||||
| Linkage group(s) | |||||
| Developed by | HiDRAS consortium | ||||
| Other maps | Discovery-5 Fiesta x Totem-5 Apple Integrated Map-5 M.27 x M.116-5 | ||||
| Reference publication | Silfverberg-Dilworth E, Matasci C L, Van de Weg W E, Van Kaauwen M P W,Walser M, Kodde L P, Soglio V, Gianfranceschi L, Durel C E, Costa F, Yamamoto T, Koller B, Gessler C, Patocchi A (2006) Microsatellite markers spanning the apple (Malus x domestica Borkh) genome. Tree Genetics & Genomes 2(4): 202-224 | ||||
| Remarks | clean | ||||
| Sequence | |||||
| >Hi22f12 AAGATGGAACAATATTTACATGTAATTGGAGCGGCCTCACCCAGTCTACATTATCTTTCT TCTTCTTCTTCTTCTTCTGCTTCTTCTTTCTCGTTAGTTTCAACATTAAACTTTGTGTCC TCTGAGAACTCTGGAGCTGCTGCAGAACTGCCACTGCCATCCATCTTCACCTCCATGTTT TGAAGGACGTCATCTCGAGAAGCGAGCTCGTCAATCTCAGGCAAAGTACCCCATCACACC TTTCACAGTGGAAGTACCCATCTTCCAATTTATCTTTCAGTCTCAAACAATCTATATTTG ACTTGAGCACTATGAGAGATTATGCACTAG | |||||
| User's notes | |||||
| THERE ARE NO USER'S NOTES. | |||||
| Allele size and gel image | |||||
| Cultivar | Allele size |  | |||
|
Fiesta Discovery Gala Florina Nova Easygro TN10-8 Durello di Forli Prima Modial Gala Fuji |
211 211:217 211 214:217 205 217 205 217 |
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