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Hi22f12
Primer features
Name Sequence Length Tm  
Hi22f12.F GGCCTCACCCAGTCTACATT 20 nt 60.5 °C Primer Stats
Hi22f12.R GTTTGGTGTGATGGGGTACTTTGC 24 nt 65.2 °C
SSR info
SSR repeat type Imperfect
Locus type single locus
Detected loci
Alleles size range 205-217
Number of alleles detected 4
Expected heterozygosity n.d.
PCR annealing temp 60
Sequenced allele size 0
Linkage group(s)
Developed by HiDRAS consortium
Other maps Discovery-5 Fiesta x Totem-5 Apple Integrated Map-5 M.27 x M.116-5
Reference publication Silfverberg-Dilworth E, Matasci C L, Van de Weg W E, Van Kaauwen M P W,Walser M, Kodde L P, Soglio V, Gianfranceschi L, Durel C E, Costa F, Yamamoto T, Koller B, Gessler C, Patocchi A (2006) Microsatellite markers spanning the apple (Malus x domestica Borkh) genome. Tree Genetics & Genomes 2(4): 202-224
Remarks clean
Sequence
>Hi22f12 AAGATGGAACAATATTTACATGTAATTGGAGCGGCCTCACCCAGTCTACATTATCTTTCT TCTTCTTCTTCTTCTTCTGCTTCTTCTTTCTCGTTAGTTTCAACATTAAACTTTGTGTCC TCTGAGAACTCTGGAGCTGCTGCAGAACTGCCACTGCCATCCATCTTCACCTCCATGTTT TGAAGGACGTCATCTCGAGAAGCGAGCTCGTCAATCTCAGGCAAAGTACCCCATCACACC TTTCACAGTGGAAGTACCCATCTTCCAATTTATCTTTCAGTCTCAAACAATCTATATTTG ACTTGAGCACTATGAGAGATTATGCACTAG
User's notes
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Allele size and gel image
Cultivar Allele size
Fiesta
Discovery
Gala
Florina
Nova Easygro
TN10-8
Durello di Forli
Prima
Modial Gala
Fuji
211
211:217
211
214:217
205
217

205

217


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