IPPN14
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| IPPN14.F | GAGGAAGTAACCGCATCAGC | 20 nt | 60.5 °C |  |
|
| IPPN14.R | (GTTTCTT)TCTAAGGGCAGGCAGATCAC | 29 nt | 69.0 °C | ||
| SSR info | |||||
| SSR repeat type | unknown | ||||
| Locus type | |||||
| Detected loci | |||||
| Alleles size range | 223-252 | ||||
| Number of alleles detected | 0 | ||||
| Expected heterozygosity | 0.66 | ||||
| PCR annealing temp | 60 | ||||
| Sequenced allele size | 0 | ||||
| Linkage group(s) | |||||
| Developed by | College of Agriculture, Ibaraki University, Ibaraki, Japan | ||||
| Other maps | Fiesta x Totem-11 M.27 x M.116-11 | ||||
| Reference publication | Inoue E., Matsuki Y., Anzai H., Evans K.M. (2007) Isolation and characterization of microsatellite markers in Japanese pear (Pyrus pyrifolia Nakai). Molecular Ecology Notes 7(3): 445-447 | ||||
| Remarks | SSR marker developed in pear | ||||
| Sequence | |||||
| >gi|125995317|dbj|AB250793.1| Pyrus pyrifolia DNA, microsatellite locus IPPN014 ACACACACACACATATATATGATCGATGGGTTTACTTACAGAGCCTGGTGTATAGGAGGATATTGGTGGG ACGCTGTGCACTTGGACGCTTTCCATAAGGAGTCGTTGGTAGGGATCGCAAGACAAGTAGAGGTTCTTAA GTAGGACTAGTTGTTCCTTTGAGGAAGTAACCGCATCAGCTGGCTGATCAGTCTCTGCGGGAAGCTTCAA CTTGATAGTAGCGGTGTTGATCACACACAAGTCTGACTCTTTGGGAAAAGCCTTGACTACATAATCCCTC AGTATCACCTGCTTGTTACTCACTTCTGCCATTGCTCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC TCTCTCTCTCTCTCTCTAATCAATATCTTTTTACGTGATCTGCCTGCCCTTAGAGATCGACCAAACCTTA TATATAGCAGTGGAGGACGTTCTACTAGAGCAACAGCTTTCCTTAATTTGAGCTAAAG | |||||
| User's notes | |||||
| THERE ARE NO USER'S NOTES. | |||||
| Allele size and gel image | |||||
| Cultivar | Allele size | ||||
|
Not available |
|||||
Send comments to Luca Gianfranceschi
