IPPN15
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| IPPN15.F | GAAGCACTCAATGGGGAAAA | 20 nt | 56.4 °C |  |
|
| IPPN15.R | (GTTTCTT)AACCATGGCATTGGATTGAT | 29 nt | 64.8 °C | ||
| SSR info | |||||
| SSR repeat type | unknown | ||||
| Locus type | |||||
| Detected loci | |||||
| Alleles size range | 182-265 | ||||
| Number of alleles detected | 0 | ||||
| Expected heterozygosity | MP | ||||
| PCR annealing temp | 60 | ||||
| Sequenced allele size | 0 | ||||
| Linkage group(s) | |||||
| Developed by | College of Agriculture, Ibaraki University, Ibaraki, Japan | ||||
| Other maps | Fiesta x Totem-3 M.27 x M.116-3 | ||||
| Reference publication | Inoue E., Matsuki Y., Anzai H., Evans K.M. (2007) Isolation and characterization of microsatellite markers in Japanese pear (Pyrus pyrifolia Nakai). Molecular Ecology Notes 7(3): 445-447 | ||||
| Remarks | SSR marker developed in pear | ||||
| Sequence | |||||
| >gi|125995318|dbj|AB250794.1| Pyrus pyrifolia DNA, microsatellite locus IPPN015 TCTTGCTTACGCGTGGNACTAACATTTGAAGAGAAGCACTCAATGGGGAAAAGAGGTCTCTCTCTTTCTT TCTCTCTCTCTCTCTCTCTCTCTCTTTCTCTCTCTCTCTCTCTCACATACACACACACACACACACACAC ACACACACGCACACACACAACTGGACACTAATTTGAGCTTGATGAATGTCTATAATTTGCAGATGGGATT GAAATATGGATGTCCGGTAGAAGATGTGATCACAGGGCTATCAATCCAATGCCATGGTTGGAAATCAGTG TATTGCAATCCCACAAGGAAAGCTTTCTTAGGAGTAGCCCCAACTACGCTAACTCAGACGCTTATGCAGC ATAAGAGATGGACAGAAGGCGAGTTCCAAATTTTGTTTACTAAATACAGCCCTGCGTGGTATGCACATGG AAAAATTAGCTTTGGTCTTCAACTTGGATATTGTTGCTTCTGCTTCTGGTGTTCGAATTCCTTGGCAGCG TTATTTTATTCGATTGTCCCTTCCCTTTACCTCCTCAAGGGCATTTCCTTATTTCCACAGGTTAGTCCAC GCGTAAGCAAGA | |||||
| User's notes | |||||
| THERE ARE NO USER'S NOTES. | |||||
| Allele size and gel image | |||||
| Cultivar | Allele size | ||||
|
Not available |
|||||
Send comments to Luca Gianfranceschi
