MS06g03
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| MS06g03.F | CGGAGGGTGTGCTGCCGAAG | 20 nt | 66.6 °C |  |
|
| MS06g03.R | GCCCAGCCCATATCTGCT | 18 nt | 58.4 °C | ||
| SSR info | |||||
| SSR repeat type | perfect | ||||
| Locus type | presumed multi-locus | ||||
| Detected loci | |||||
| Alleles size range | 154-190 | ||||
| Number of alleles detected | 9 | ||||
| Expected heterozygosity | 0.87 | ||||
| PCR annealing temp | 0 | ||||
| Sequenced allele size | 0 | ||||
| Linkage group(s) | |||||
| Developed by | Warwick HRI | ||||
| Other maps | Discovery-10 Fiesta-10 Fiesta-10 Fiesta x Totem-10 PI 613988-10 Royal Gala-10 Apple Integrated Map-10 M.27 x M.116-10 | ||||
| Reference publication | Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241 | ||||
| Remarks | |||||
| Sequence | |||||
| >gi|58003368|gb|AY861489.1| Malus x domestica clone MS06G03 microsatellite sequence CTTNTCTNCTGCGNNGTCTATCCTCCAACCGGAGGGTGCTGCCGAAGGAGCGAGAGAGAGAGAGAGAGAG AGANAGANAGAGAGAGAGAGAGAGAGGAAANGTAGCCGGAGCCANGTCTGGTGCTGAAAATGTAAAAACG AAAAACCCCAATTTTCCAAAACCCCAAATTCTTTGTATTCGGATCGACAAATGAGCAGATATGGGCTGGG CTTATTATTANGTATATGATGGGCTTACATTTTACGAACCCAA | |||||
| User's notes | |||||
| Luca Gianfranceschi (luca.gianfranceschi@unimi.it) Date 28 September 2005 - Time: 14:47 This SSR marker cosegregates with the enzyme Md-ACO1 (1-aminocyclopropane-1-carboxylate oxydase). Ref. Euphytica 141:1-2 181-190. LucaG (luca.gianfranceschi@unimi.it) Date 13 February 2006 - Time: 15:08 Sequence Cultivar: Fiesta syn. Red Pippin View Sequence Info at PlantGDB | |||||
| Allele size and gel image | |||||
| Cultivar | Allele size | ||||
|
Not available |
|||||
Send comments to Luca Gianfranceschi
