MS14b04
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| MS14b04.F | CCTTAAGAATCATGTGAT | 18 nt | 47.0 °C |  |
|
| MS14b04.R | ACTAATGGCACAAAGATTGT | 20 nt | 52.3 °C | ||
| SSR info | |||||
| SSR repeat type | compound | ||||
| Locus type | single locus | ||||
| Detected loci | |||||
| Alleles size range | 230-292 | ||||
| Number of alleles detected | 5 | ||||
| Expected heterozygosity | 0.75 | ||||
| PCR annealing temp | 0 | ||||
| Sequenced allele size | 280 | ||||
| Linkage group(s) | |||||
| Developed by | Warwick HRI | ||||
| Other maps | Discovery-12 Fiesta-12 Fiesta x Totem-12 | ||||
| Reference publication | Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241 | ||||
| Remarks | |||||
| Sequence | |||||
| >gi|58003358|gb|AY861479.1| Malus x domestica clone MS14B04 microsatellite sequence CCCGCCTTGTTCATCCCATACCTTAAGAACATGTGATGTGTGTGTGTGTGTGTGTGTGTGAGAGAGAGAG AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGACCTGAGAATCAGCTTCCATTGATGATTGAAGATACTTTC TGAGCATGAGAANGGTCAGGTTAATGCCTTCATTCCCTGCCAATCTATAGCAGTTAATAAAACTGAACCC CGCCCACACTGAACTGCCTGCAGACAATGGATTTGAAAACGTCTCCAGTGACGAACAATCTTGTGCCATT AGTTCTCTTACACTCGATGGAATGTCTGGCAATAATTGAACCTCTTGCAACCATTTAAGTTCAGTTCTGT AAGCTTTGTAAGTTGACTGATGCTTGCNG | |||||
| User's notes | |||||
| LucaG (luca.gianfranceschi@unimi.it) Date 13 February 2006 - Time: 15:10 Sequence Cultivar: Fiesta syn. Red Pippin View Sequence Info at PlantGDB | |||||
| Allele size and gel image | |||||
| Cultivar | Allele size | ||||
|
Not available |
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