MS14h03
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| MS14h03.F | CGCTCACCTCGTAGACGT | 18 nt | 58.4 °C |  |
|
| MS14h03.R | ATGCAATGGCTAAGCATA | 18 nt | 49.3 °C | ||
| SSR info | |||||
| SSR repeat type | perfect | ||||
| Locus type | single locus | ||||
| Detected loci | |||||
| Alleles size range | 114-140 | ||||
| Number of alleles detected | 6 | ||||
| Expected heterozygosity | 0.8 | ||||
| PCR annealing temp | 57 | ||||
| Sequenced allele size | 130 | ||||
| Linkage group(s) | |||||
| Developed by | Warwick HRI | ||||
| Other maps | Fiesta x Discovery-3 Prima x Fiesta-3 Fiesta-3 Prima-3 Discovery-3 Fiesta-3 Fiesta x Totem-3 Apple Integrated Map-3 M.27 x M.116-3 | ||||
| Reference publication | Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241 | ||||
| Remarks | |||||
| Sequence | |||||
| >gi|58003352|gb|AY861473.1| Malus x domestica clone MS14H03 microsatellite sequence TCAAGCTATANAAATTTTCTGAGCGCTCACCTCGTAGACGTTTGCGAAGCCCACCGAAAGGTGGAAGCCC TCCCAGGTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCAAGCCTGAAAGCAT ATGCTTAGCCATTGCATACACAAAACATATGAGGTGAGAAAAAGTAAAGTCAGAGCATAAATGATCTTTC TGTTAGAGACGAAAGAAATACAAACATTTTGATCATGGTAGACCGACATGTCTTTTTTCTTTTGTTTATA CACATGCAGAGAGCTAGACTCAACAACAACAACAACAACAACAAAGCCTTTTCCCACTAAGTGGGGTCGG CTATACGAATCCTANAACGCCATTGCGCTCGGTTTTGTGTTATGTCCTCCGTTAGATCCAA | |||||
| User's notes | |||||
| LucaG (luca.gianfranceschi@unimi.it) Date 13 February 2006 - Time: 15:12 Sequence Cultivar: Fiesta syn. Red Pippin View Sequence Info at PlantGDB | |||||
| Allele size and gel image | |||||
| Cultivar | Allele size | ||||
|
Not available |
|||||
Send comments to Luca Gianfranceschi
