MSS5
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| MSS5.F | CCCCAACAACATTTTTCTCC | 20 nt | 56.4 °C |  |
|
| MSS5.R | CCTCTCGCTCTTTGCCTCT | 19 nt | 59.5 °C | ||
| SSR info | |||||
| SSR repeat type | unknown | ||||
| Locus type | |||||
| Detected loci | |||||
| Alleles size range | 130-146 | ||||
| Number of alleles detected | 7 | ||||
| Expected heterozygosity | 0.72 | ||||
| PCR annealing temp | 60 | ||||
| Sequenced allele size | 138 | ||||
| Linkage group(s) | |||||
| Developed by | CGAF, ONF, Campus INRA, Ardon, France, | ||||
| Other maps | Fiesta x Totem-8 | ||||
| Reference publication | Oddou-Muratorio, S; Aligon, C; Decroocq, S; Plomion, C; Lamant, T; Mush-Demesure, B (2001) Microsatellite primers for Sorbus torminalis and related species. Molecular Ecology Notes 1(4): 297-299 | ||||
| Remarks | SSR marker developed in Sorbus torminalis | ||||
| Sequence | |||||
| >gi|20067405|emb|AJ313038.1| Sorbus torminalis microsatellite DNA, clone 32 GCTAATGGTGTTCAGATCAAAGGTGATTTCTGACATGCTTGTGGTCCTCTTAATTGGAGGCCTTGGTCTC TTCCCCAACAACATTTTTCTCCTCTCTGCATATACTACTAACAACCTGAAAAGGCTACAAACTGCTATTC TCTCTAAATCTCTGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGCAAAGAGCGAGAGG GGGGTGAAGCGAAGGTGGGGAAGATAGAGAAGAGCCAGAAACTGAGGAGCGAATCTGGACCGTTGGTTGT TGCAGTTCTTAATGGAGATTGAGGGTTGTGGAGTCTTGTGACCTGTGACTTGTGATTTGTAAGAGTGAGC TGTTTGCATGAATGGAAAATCCACGCCAGCACTTTAATTATTTTATTTTATTTGTTTAATACTAAGGCAA ATGGAGCTTTGGATGCTTTTTATTTATTTATTTTACCTTCTAAAGCTTGAAGTCACTTACAAAGAGTTTT ACATGCTCTCCATTTGACACGCAGAACTTATGTTGATTATAGCCCTTTGTAATTGCTTTGAGCGTGAATG AAGCTTACTTGTAGTTGATGACTAATCTTAGGATCTTGGTTC | |||||
| User's notes | |||||
| THERE ARE NO USER'S NOTES. | |||||
| Allele size and gel image | |||||
| Cultivar | Allele size | ||||
|
Not available |
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