U78949-SSR
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| U78949-SSR.F | TTTGTCTACCTCTGATCTTAACCAA | 25 nt | 60.9 °C |  |
|
| U78949-SSR.R | CAGCATCGCAGAGAACTGAG | 20 nt | 60.5 °C | ||
| SSR info | |||||
| SSR repeat type | Perfect | ||||
| Locus type | multi-locus | ||||
| Detected loci | |||||
| Alleles size range | 172-225 | ||||
| Number of alleles detected | 12 | ||||
| Expected heterozygosity | n.d. | ||||
| PCR annealing temp | 60 | ||||
| Sequenced allele size | 0 | ||||
| Linkage group(s) | |||||
| Developed by | HiDRAS consortium | ||||
| Other maps | Discovery-6 Discovery-14 Fiesta-6 Apple Integrated Map-14 | ||||
| Reference publication | Silfverberg-Dilworth E, Matasci C L, Van de Weg W E, Van Kaauwen M P W,Walser M, Kodde L P, Soglio V, Gianfranceschi L, Durel C E, Costa F, Yamamoto T, Koller B, Gessler C, Patocchi A (2006) Microsatellite markers spanning the apple (Malus x domestica Borkh) genome. Tree Genetics & Genomes 2(4): 202-224 | ||||
| Remarks | extra bands | ||||
| Sequence | |||||
| >gi|5777903|gb|U78949.1|MDU78949 Malus domestica MADS-box protein 3 mRNA, complete cds AGTTTGTCTACCTCTGATCTTAACCAAGTAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA GAGAGAGAGAGATGGGCAGAGGCAGAGTTGAGCTGAAGAGGATAGAGAATAAAATTAACAGGCAAGTGAC TTTTGCCAAAAGAAGAAATGGGCTGCTCAAGAAAGCTTATGAGCTCTCAGTTCTCTGCGATGCTGAGGTT GCCCTCATTATTTTCTCTAGCCGTGGCAAGCTCTATGAATTCTGTAGCAGTTTCAGCATGATGAAAACGC TTGAAAAGTACCAAAGTTGCAGTTATGGTTCCCTGGAAGCCAACCTACCAGCCAACGAGACCCAGAACAG CTATCAGGACTATTTGATGTTGAAGGCAAGAGTTGAGGTCCTCCAACAATCTCAAAGAAACCTTCTTGGG GAAGATTTGTCCCACCTGAACACAAAGGAGCTTGAGCATCTTGAGCATCAATTGGAGACCTCTTTGAAGC AAATTAGGTCAAGAAAGACTCAGTTTATTCTCGATCAGCTTTCTGATCTTCAAAACAGGGAACAGATGCT AGTTGAAGCTAACAAAGCCTTGAAGAGGAAGCTGGAAGAAACAAGTGTGCAAGCTCCAGAAGGAATGGCA TGGGAAGCTGCTGGCCATGGCCCCAACAACATTCAGCAAACTCGGCTTCCTTCCCACTCAGAAGCCTTCT TCCATCCCTTGGAAGGAAACAACTCCAGTTCCCAAATTGGATACACCCATATGGGTTCAGATAATGAAAT GAATGTTGGAAATCCGGGCCAGTATGTTAATGGATATATTCCTGGGTGGATGCTTTGACTTTGATTATGC ATATAATATCCATCGTGACATATAATATACATATACATATACATATACATATATATATATATGTATATGT ATGGAATAAAATTAGCAAGGGATCATGTTGATCAAGCTTGTATATTATTTTCTGTTATGATCTTATAATT CTATGCAACTTGTAATGATTCTTGAAACAATAAGAATAATATATATATATATATATAAGTTTTGTAAGTT GAATTAATGTCATTGAAAAACTTCTATGAAATGTATGAGACCAATTTGTATATA | |||||
| User's notes | |||||
| LucaG (luca.gianfranceschi@unimi.it) Date 13 February 2006 - Time: 14:25 Sequence Assembly Library Reference View Sequence Info at PlantGDB | |||||
| Allele size and gel image | |||||
| Cultivar | Allele size |  | |||
|
Fiesta Discovery Gala Florina Nova Easygro TN10-8 Durello di Forli Prima Modial Gala Fuji |
176:209:215:225 174:209:221:225 180:190:205 176:205:219 174:219 176:207:219 174:215 190:205 |
||||
Send comments to Luca Gianfranceschi
