Z38126-SSR
Primer features | |||||
Name | Sequence | Length | Tm | ||
Z38126-SSR.F | AAGAGGGTGTTCCCAGATCC | 20 nt | 60.5 °C | ||
Z38126-SSR.R | TGTTCGATGTGACTTCAATGC | 21 nt | 57.4 °C | ||
SSR info | |||||
SSR repeat type | Perfect | ||||
Locus type | single locus | ||||
Detected loci | |||||
Alleles size range | 214-240 | ||||
Number of alleles detected | 3 | ||||
Expected heterozygosity | n.d. | ||||
PCR annealing temp | 60 | ||||
Sequenced allele size | 0 | ||||
Linkage group(s) | |||||
Developed by | HiDRAS consortium | ||||
Other maps | Discovery-7 Apple Integrated Map-7 | ||||
Reference publication | Silfverberg-Dilworth E, Matasci C L, Van de Weg W E, Van Kaauwen M P W,Walser M, Kodde L P, Soglio V, Gianfranceschi L, Durel C E, Costa F, Yamamoto T, Koller B, Gessler C, Patocchi A (2006) Microsatellite markers spanning the apple (Malus x domestica Borkh) genome. Tree Genetics & Genomes 2(4): 202-224 | ||||
Remarks | clean | ||||
Sequence | |||||
>gi|984159|emb|Z38126.1|MDCALBPK M.domestica gene for calmodulin-binding protein kinase GGATCCTCATTTTTCTAGAAAAATTGGGACATCCTCTTAACCGTTGGATTTGACTTTTGAAATTGTGTGG TTGAGATTGTATGGCCTGTGATTTAATTTTAACCACACAATTTCATTAAAGTCAATCCAACGGTTAAGAG GGTGTTCCCAGATCCCCCTTGTTAAAGGACCTATATATATATATATGACCATTAATAAGTGCAAACTCCA TAATCGATACGATTTTATGATATGGAAACTAAATAGCTGATATTAAAAATTAAAGATCTAAATAGTTGTA AAAGTAAAATTTAACAGCTAAAATACTCGTACCATTATATGATATGTAGCATTGAAGTCACATCGAACAG ACAGATAGATATACATACATACATACAAAACCTACTACAAAAATGACATGCGTGCATGACATACATGGTT ATTATTAGTTGACTGTGTCTGTCTTTTCTTCCTCACGAATTAACTTGGACAATATATTCCCTCCCTCCCC GTATGAATAATTCCACCCATATTTTATGCATTAAACAAGTTGAAGAAACTGCTCGCAAGCTGATGTCAAA CGATACCAACTTGTCCTTTCGACAATATTAAAAACACCATTTGTCTCTGCAAGACTACTTCCTTCCTTCC CTCCTCATCAGCTTCCTTAAGATTCCATCTCTTCCCTCTTGGTTCGGTCTCTGCTTTGCCTGTTCGATTT CATCAAGGTTTGTTTGTCTTTGTCTTCATTGGAATCGTAACCATGATACAAGAAACAAGAAGACTTGCAG ATGAGTATGAGATATCAGAGATTTTAGGCAGAGGAGGATTCTCTGTGGTCAGAAAAGGCATCAGCAGAAA GTCAAGCAGCAGCAGTGACAAAACCGATGTGGCAATCAAAACACTCAAAAGGCCGTTTGCACCCTCGAAC CCTCCTCCTCTGCCGCCCCACGCCCGGCGGAACGACCAGAATAGCTTTGCTGCTGCCGCTTTCCAAACCC GGAAGCAGGTGTCCATATCGAATGTGCTGCTTACAAATGAAATCCTGGTGATGAGGAAGATTGTTGAGAA TGTGTCGCCGCACCCGAATGTGATTGACCTCTATGATGTGTACGAGGATGAGAACGGGGTTCACCTTGTG CTGGAGCTATGTTCTGGAGGGGAACTGTTTGATAGGATTGTGAAGCAAGAAAGGTATTCTGAGGTTGGAG CTGCAGCTGTGGTGAGGCAGATTGCCCAAGGCTTGGCGGCTCTTCACCGCTCGAATATTGTTCACCGGGA CTTGAAGCCGGAAAACTGCCTCTTCTTGGACAACACCGTTGATTCTCCGTTGAAGATTATGGATTTTGGG CTGAGTTCCGTGGAGGAGTTCACTGACCCTGTTGTGGGACTGTTTGGTTCCATAGACTATGTGTCACCAG AGGCTCTTTCTCAGGGGCAAGTAACTTCCAAGAGCGATATGTGGGCTCTTGGTGTAATCTTATATATCCT CCTCTCCGGGTATGTTCATATTTTTCTCTTGATATTATGGGCAACCAAACAGAAGACGGTAGCATTAGTA GCGTATGAAGCATATTACAACACAGAAATAATATGATTTGCTTTTTAGGTACCCACCTTTTATTGCTCAG TCGAATCGGCAAAAGCAACAGATGATTATGGCTGTAAGCAATTAGCATTGACATTCTATTAGAACACATA AGTTAGTATGTTTTGATTTGTCAAAAGGTTCGTTCAAATTAAAAGAGAAAATGCTTGTGGTCCTGCAGGG AGAATTCAGTTTCTATGAGAAAACCTGGAAGGGATTTCTTTGTCAGCCAAAGCAATTGATTTCGAGCCTC CTCAAAGTCGACCCTGACAAGAGACCTAGTGCTCAAGAGGTATGCTTGGAAATCTATGCAAAAGATTAAG CATCAACAAACACAACTAAAATATCGAGAGAATGTCAACATCGGTCTGAGCTATATAGAAACTAAATGAA CTCTTATAGTAGTTCTTGGTAGCACCAAAATGCAGTCTCCTAGAATTGTTAGCCTAACTAATTTATTTCT TGTGTTAAACAGCTTCTGGACCACCCATGGGTTGTCGGTCTTTCAGCCAGAGAGGATCAAATGGATGCTG AGATTGTATCACGACTGCAGAGTTTTAATGCTCGGCGCAAACTCCGTGCTGCAGCAATAGCAAGCGTGTG GACAAGCTCGATTTTCTTAAGGACAAAGAAGCTAAAATCGTTACTAGGATCCTATGACCTTAAACCAGAC GAAATCAAGAATCTGAGTTCACATTTTAAGAAAATGTGAGTAGTTAAAAGCACATTTTGCACCTTCCTAT ATTGTCTATGAATGTGGTATTAAAATGTAGGTCAGACCGAGAATTATCAGTACTTAGCATCCAGTTTCTG CTAAAAGTTTGCATATATAGTAATAGCAGTTATATGGATCACTTCTCTACGTAAGTTCAGTTTAATGACT AAGACTTATTGAGATAACAAACGAAAAATGGCATTCTTTTTAAGTGATAAATTCACTTGGGCAATCCTAA ACTACACTGCTAGAGCCTAAGCTTAGTTCTCCTGTAATCCAATATTTATAAATTATGATCAACTAATATT TCAAAGTACAGATGTGTAAAAGGTGACAATGCTACTCTTTCTGAATTCAGGTTGAGTTGCAAGCGACTGA TCCAACTGAGATTGGTCTCGATGTCATTAAGAGCCGAGCCAGCTAGATTATGAATCATAGGCTATCTGGT GGGGAGTTCGTCCCTGCACCGTTTGTTACTTGCGTGATATGTTTATACATTGTGGAAATGGCGACTTGGC GAAAGATGGAATCAATTTCCAAGTTTTGCCGTTGTTTCTTATTTATTTTAAACAAGCGTCTGTGGTTTCT GGTATATCATATGATATGTTTATTTGATAGAACCTATCTTTGTTGAGTGGCTGCTTTAGTAGCTGTATGT CAACAAACTAATATGTAGCTGTTTGTGGGGTTTAGACGGCACCTTTCCGCCTTCCGAGTGTTGTATATGA CCCTTGTCATTTTACTTTGGAGCAGTATAAAAGGAGCATGCTTTTATGGCCTTTCCTTGTTGCT | |||||
User's notes | |||||
LucaG (luca.gianfranceschi@unimi.it) Date 13 February 2006 - Time: 14:27 Sequence Library Reference View Sequence Info at PlantGDB | |||||
Allele size and gel image | |||||
Cultivar | Allele size | ||||
Fiesta Discovery Gala Florina Nova Easygro TN10-8 Durello di Forli Prima Modial Gala Fuji |
216 214 214 214:216 214 214 214 214:240 |
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