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CH01a09
Primer features
Name Sequence Length Tm  
CH01a09.F GATGTGGTTCCAGAAGCTAC 20 nt 58.4 °C Primer Stats
CH01a09.R CACATGCATGAAAAGCATAT 20 nt 52.3 °C
SSR info
SSR repeat type Compound
Locus type presumed multi-locus
Detected loci
Alleles size range 198-384
Number of alleles detected 10
Expected heterozygosity n.d.
PCR annealing temp 55
Sequenced allele size 198
Linkage group(s)
Developed by ETH Zuerich
Other maps Fiesta x Totem-14
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH01a09 tagtctgcaaagaaggtaaccactgaagatgatgtggttccagaagctactcctgctatactgtataggtactctcatat tctgttattgtctttgtctaaaactcttgattttttagtaaatggcgatctctctctctctctctctctctctctctctc tcacacacacacacacacacacacacgtgtgtgtgngcgcgcatgatnatatgcttttcatgcatgtgnatctgcatgta ntntgtggnatactaagcacatgctcccnacacttgtntntgtgttctgtgttctgtctgtgtgccggaaaacaaaatat ctacttatatttaatctctggtataatcctccatanggtgcctaatt
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
204
198:204
200:204:360

204:210:276
200:204:360
200:204:360
200:204:360:370


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