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CH01h01
Primer features
Name Sequence Length Tm  
CH01h01.F GAAAGACTTGCAGTGGGAGC 20 nt 60.5 °C Primer Stats
CH01h01.R GGAGTGGGTTTGAGAAGGTT 20 nt 58.4 °C
SSR info
SSR repeat type Perfect
Locus type single locus
Detected loci
Alleles size range 114-134
Number of alleles detected 6
Expected heterozygosity 0.8
PCR annealing temp 60
Sequenced allele size 133
Linkage group(s)
Developed by ETH Zuerich
Other maps Braeburn-2 Telamon-2 Discovery-17 Fiesta-17 Fiesta x Totem-17 Royal Gala-17 Apple Integrated Map-17 M.27 x M.116-17
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH01h01 agccccatgtccagttgcctagtctctattaacgaggatttccgtagtggcctcacaagtgtcccgaaagacttgcagtg ggagcagtttctcaaggagaccaacaactgtctcgtgcctccattctctctctctctctctctctctctctctctctctc tctctctctctctctataaaccttctcaaacccactcctaaagtgatcaacccaactgtcanggtggttactagttcttc aacntctctccntcacattntctctctntcccattattcctccaagctttatctgcaaatnaagatggtnttcattgcta ccattttgttctntcaccttcaaaacagcccaaaatt
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
118:122
122:134
122:134

124
120:134
120:124
120


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