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CH02d08
Primer features
Name Sequence Length Tm  
CH02d08.F TCCAAAATGGCGTACCTCTC 20 nt 58.4 °C Primer Stats
CH02d08.R GCAGACACTCACTCACTATCTCTC 24 nt 65.2 °C
SSR info
SSR repeat type Perfect
Locus type single locus
Detected loci
Alleles size range 210-254
Number of alleles detected 7
Expected heterozygosity 0.82
PCR annealing temp 60
Sequenced allele size 231
Linkage group(s)
Developed by ETH Zuerich
Other maps Discovery-11 Discovery x TN10-8-11 Discovery-11 Fiesta-11 Fiesta x Totem-11 PI 613988-11 Royal Gala-11 Malling9-11 Apple Integrated Map-11 M.27 x M.116-11
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH02d08 aattagtctttgattgctttgctcttccaaaatggcgtacctctctcattccctcatcaggcttcatcaacagcaaccat tcctcttttatttctttgctctcccttttgccaccaccatatgcataatgcatatgcacattttctactcacattaactg cttttgcagtctacgtccatggctaatatcaaatatcgagagagagagagagagagagagagagagagagagagagatag tgagtgagtgtctgctttaatt
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
254
224:254
228:250

228:254
212:228
212:254
212:218


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