CH02d08
Primer features | |||||
Name | Sequence | Length | Tm | ||
CH02d08.F | TCCAAAATGGCGTACCTCTC | 20 nt | 58.4 °C | ||
CH02d08.R | GCAGACACTCACTCACTATCTCTC | 24 nt | 65.2 °C | ||
SSR info | |||||
SSR repeat type | Perfect | ||||
Locus type | single locus | ||||
Detected loci | |||||
Alleles size range | 210-254 | ||||
Number of alleles detected | 7 | ||||
Expected heterozygosity | 0.82 | ||||
PCR annealing temp | 60 | ||||
Sequenced allele size | 231 | ||||
Linkage group(s) | |||||
Developed by | ETH Zuerich | ||||
Other maps | Discovery-11 Discovery x TN10-8-11 Discovery-11 Fiesta-11 Fiesta x Totem-11 PI 613988-11 Royal Gala-11 Malling9-11 Apple Integrated Map-11 M.27 x M.116-11 | ||||
Reference publication | Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241 | ||||
Remarks | none | ||||
Sequence | |||||
>CH02d08 aattagtctttgattgctttgctcttccaaaatggcgtacctctctcattccctcatcaggcttcatcaacagcaaccat tcctcttttatttctttgctctcccttttgccaccaccatatgcataatgcatatgcacattttctactcacattaactg cttttgcagtctacgtccatggctaatatcaaatatcgagagagagagagagagagagagagagagagagagagagatag tgagtgagtgtctgctttaatt | |||||
User's notes | |||||
THERE ARE NO USER'S NOTES. | |||||
Allele size and gel image | |||||
Cultivar | Allele size | ||||
Prima Fiesta Discovery Durello di Forlí TN10-8 Florina Nova Easygro Starking |
254 224:254 228:250 228:254 212:228 212:254 212:218 |
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