CH03a03
Primer features | |||||
Name | Sequence | Length | Tm | ||
CH03a03.F | GTGGTGGTAATGACGAGAACCT | 22 nt | 62.1 °C | ![]() |
|
CH03a03.R | AAGCAAAGTAGCCAAACTGCAT | 22 nt | 58.4 °C | ||
SSR info | |||||
SSR repeat type | Perfect | ||||
Locus type | presumed single locus | ||||
Detected loci | |||||
Alleles size range | 154-182 | ||||
Number of alleles detected | 7 | ||||
Expected heterozygosity | 0.81 | ||||
PCR annealing temp | 60 | ||||
Sequenced allele size | 168 | ||||
Linkage group(s) | |||||
Developed by | ETH Zuerich | ||||
Other maps | Fiesta x Totem-14 M.27 x M.116-14 | ||||
Reference publication | Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241 | ||||
Remarks | none | ||||
Sequence | |||||
>CH03a03 aattgttagaatacttaagaagcctacaaccttgccactataccacttctactctaaccctttttattgttacatgccat ggttggtctccaacaaagcaatgcgttctttttcaggtcgcaccctgtgatacatgcggtggtggtaatgacgagaacct cactgcaacttgccgtaaatgcaatatagctcgtgcacacccgtatgactctctctctctctctctctctctctctctct ctctctctctgcacaagcatatatttagctgcatttatgtgtgcatgcagtttggctactttgcttgcctgtcaatcaag aactgaatagtatgtcttcttaataatatcagttgatcaaaatcatgatgcatcttgtnttttgccttttttttcctttg aaaatt | |||||
User's notes | |||||
THERE ARE NO USER'S NOTES. | |||||
Allele size and gel image | |||||
Cultivar | Allele size | ![]() | |||
Prima Fiesta Discovery Durello di Forlí TN10-8 Florina Nova Easygro Starking |
166:182 170:170 154:170 154:170 156:170 168:182 156:168 |

