CH03d08
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| CH03d08.F | CATCAGTCTCTTGCACTGGAAA | 22 nt | 60.3 °C |  |
|
| CH03d08.R | TAGGGCTAGGGAGAGATGATGA | 22 nt | 62.1 °C | ||
| SSR info | |||||
| SSR repeat type | Perfect | ||||
| Locus type | single locus | ||||
| Detected loci | |||||
| Alleles size range | 129-161 | ||||
| Number of alleles detected | 5 | ||||
| Expected heterozygosity | 0.71 | ||||
| PCR annealing temp | 60 | ||||
| Sequenced allele size | 144 | ||||
| Linkage group(s) | |||||
| Developed by | ETH Zuerich | ||||
| Other maps | Discovery-14 Fiesta-14 Fiesta x Totem-14 Apple Integrated Map-14 M.27 x M.116-14 | ||||
| Reference publication | Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241 | ||||
| Remarks | none | ||||
| Sequence | |||||
| >CH03d08 aattagcacagtcacacttctctctcaactgcttacttcatcagtctcttgcactggaaaagatagaatacaacagaagc ctttcaagtttcaactgatcatctctctctctctctctctctctctctctctctctctctaagcttagtttcgatcatta tcatcatctctccctagccctagcttactctgtctatcttttgtggtccacaatatattcatcacctttaaaccctgcat acccatataacgtgtcgatcatttaaaacaagaaggcagcaggaaagcataaccaatt | |||||
| User's notes | |||||
| THERE ARE NO USER'S NOTES. | |||||
| Allele size and gel image | |||||
| Cultivar | Allele size | ||||
|
Prima Fiesta Discovery Durello di Forlí TN10-8 Florina Nova Easygro Starking |
129:145 133:135 133:145 129 133:161 145 133:145 |
||||
Send comments to Luca Gianfranceschi
