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CH03d08
Primer features
Name Sequence Length Tm  
CH03d08.F CATCAGTCTCTTGCACTGGAAA 22 nt 60.3 °C Primer Stats
CH03d08.R TAGGGCTAGGGAGAGATGATGA 22 nt 62.1 °C
SSR info
SSR repeat type Perfect
Locus type single locus
Detected loci
Alleles size range 129-161
Number of alleles detected 5
Expected heterozygosity 0.71
PCR annealing temp 60
Sequenced allele size 144
Linkage group(s)
Developed by ETH Zuerich
Other maps Discovery-14 Fiesta-14 Fiesta x Totem-14 Apple Integrated Map-14 M.27 x M.116-14
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH03d08 aattagcacagtcacacttctctctcaactgcttacttcatcagtctcttgcactggaaaagatagaatacaacagaagc ctttcaagtttcaactgatcatctctctctctctctctctctctctctctctctctctctaagcttagtttcgatcatta tcatcatctctccctagccctagcttactctgtctatcttttgtggtccacaatatattcatcacctttaaaccctgcat acccatataacgtgtcgatcatttaaaacaagaaggcagcaggaaagcataaccaatt
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
129:145
133:135
133:145

129
133:161
145
133:145


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