CH03d08
Primer features | |||||
Name | Sequence | Length | Tm | ||
CH03d08.F | CATCAGTCTCTTGCACTGGAAA | 22 nt | 60.3 °C | ||
CH03d08.R | TAGGGCTAGGGAGAGATGATGA | 22 nt | 62.1 °C | ||
SSR info | |||||
SSR repeat type | Perfect | ||||
Locus type | single locus | ||||
Detected loci | |||||
Alleles size range | 129-161 | ||||
Number of alleles detected | 5 | ||||
Expected heterozygosity | 0.71 | ||||
PCR annealing temp | 60 | ||||
Sequenced allele size | 144 | ||||
Linkage group(s) | |||||
Developed by | ETH Zuerich | ||||
Other maps | Discovery-14 Fiesta-14 Fiesta x Totem-14 Apple Integrated Map-14 M.27 x M.116-14 | ||||
Reference publication | Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241 | ||||
Remarks | none | ||||
Sequence | |||||
>CH03d08 aattagcacagtcacacttctctctcaactgcttacttcatcagtctcttgcactggaaaagatagaatacaacagaagc ctttcaagtttcaactgatcatctctctctctctctctctctctctctctctctctctctaagcttagtttcgatcatta tcatcatctctccctagccctagcttactctgtctatcttttgtggtccacaatatattcatcacctttaaaccctgcat acccatataacgtgtcgatcatttaaaacaagaaggcagcaggaaagcataaccaatt | |||||
User's notes | |||||
THERE ARE NO USER'S NOTES. | |||||
Allele size and gel image | |||||
Cultivar | Allele size | ||||
Prima Fiesta Discovery Durello di Forlí TN10-8 Florina Nova Easygro Starking |
129:145 133:135 133:145 129 133:161 145 133:145 |
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