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CH04a12
Primer features
Name Sequence Length Tm  
CH04a12.F CAGCCTGCAACTGCACTTAT 20 nt 58.4 °C Primer Stats
CH04a12.R ATCCATGGTCCCATAAACCA 20 nt 56.4 °C
SSR info
SSR repeat type Imperfect
Locus type single locus
Detected loci
Alleles size range 158-196
Number of alleles detected 8
Expected heterozygosity 0.86
PCR annealing temp 60
Sequenced allele size 171
Linkage group(s)
Developed by ETH Zuerich
Other maps Discovery x TN10-8-11 Discovery-11 Fiesta-11 Fiesta x Totem-11 PI 613988-11 Royal Gala-11 Malling9-11 Apple Integrated Map-11 M.27 x M.116-11
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH04a12 aattgttttacagcctgcaactgcacttatggtacttactctccctctctctctctctctctctctctctctctcttaca cacacacacacacgcacacatcagtcaaccgatccactttacatagaacaatgataaatcatgttgttttttaaagctga ttggtttatgggaccatggattttgatttactgtgagaatcctgcttacggtcatcccatctgtgacttatatgttattg acgcacccataatctattccacaaagaatt
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
172:176
164:183
164:196

172:180
178:180
172:176
176:178


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