CH04a12
Primer features | |||||
Name | Sequence | Length | Tm | ||
CH04a12.F | CAGCCTGCAACTGCACTTAT | 20 nt | 58.4 °C | ||
CH04a12.R | ATCCATGGTCCCATAAACCA | 20 nt | 56.4 °C | ||
SSR info | |||||
SSR repeat type | Imperfect | ||||
Locus type | single locus | ||||
Detected loci | |||||
Alleles size range | 158-196 | ||||
Number of alleles detected | 8 | ||||
Expected heterozygosity | 0.86 | ||||
PCR annealing temp | 60 | ||||
Sequenced allele size | 171 | ||||
Linkage group(s) | |||||
Developed by | ETH Zuerich | ||||
Other maps | Discovery x TN10-8-11 Discovery-11 Fiesta-11 Fiesta x Totem-11 PI 613988-11 Royal Gala-11 Malling9-11 Apple Integrated Map-11 M.27 x M.116-11 | ||||
Reference publication | Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241 | ||||
Remarks | none | ||||
Sequence | |||||
>CH04a12 aattgttttacagcctgcaactgcacttatggtacttactctccctctctctctctctctctctctctctctctcttaca cacacacacacacgcacacatcagtcaaccgatccactttacatagaacaatgataaatcatgttgttttttaaagctga ttggtttatgggaccatggattttgatttactgtgagaatcctgcttacggtcatcccatctgtgacttatatgttattg acgcacccataatctattccacaaagaatt | |||||
User's notes | |||||
THERE ARE NO USER'S NOTES. | |||||
Allele size and gel image | |||||
Cultivar | Allele size | ||||
Prima Fiesta Discovery Durello di Forlí TN10-8 Florina Nova Easygro Starking |
172:176 164:183 164:196 172:180 178:180 172:176 176:178 |
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