publications

(Last 5 years)


PCSK9 deficiency rewires heart metabolism and drives heart failure with preserved ejection fraction

Da Dalt L, Castiglioni L, Baragetti A, Audano M, Svecla M, Bonacina F, Pedretti S, Uboldi P, Benzoni P, Giannetti F, Barbuti A, Pellegatta F, Indino S, Donetti E, Sironi L, Mitro N, Catapano AL, Norata GD. Eur Heart J (2021) 42, 3078–3090, doi:10.1093/eurheartj/ehab431.



The funny current_even funnier than 40 years ago. uncanonical expression and roles of HCN-f channles all over the body

Benzoni P, Bertoli G, Giannetti F, Piantoni C, Milanesi R, Pecchiari M, Barbuti A, Baruscotti M, Bucchi A. Prog Biophys Mol Biol. 2021 Aug 14:S0079-6107(21)00100-0. doi: 10.1016/j.pbiomolbio.2021.08.007. Online ahead of print.



Age-Related Changes in Cardiac Autonomic Modulation and Heart Rate Variability in Mice

Piantoni C, Carnevali L, Molla D, Barbuti A, DiFrancesco D, Bucchi A and Baruscotti M. Front. Neurosci., 17 May 2021 doi.org/10.3389/fnins.2021.617698


Dual Role of miR-1 in the development and function of sinoatrial cells

Benzoni P, Nava L, Giannetti F, Guerini G, Gualdoni A, Bazzini C, Milanesi R, Bucchi A, Baruscotti M, Barbuti A. J Mol Cell Cardiol. 2021 May 6;157:104-112. doi: 10.1016/j.yjmcc.2021.05.001. Online ahead of print.


A detailed characterization of the hyperpolarization‑activated “funny” current (If) in human‑induced pluripotent stem cell (iPSC)–derived cardiomyocytes with pacemaker activity

Giannetti F, Benzoni P, Campostrini G, Milanesi R, Bucchi A, Baruscotti M, Dell’Era P, Rossini A and Barbuti A. Pflugers Arch. 2021 May 2. doi: 10.1007/s00424-021-02571-w. Online ahead of print.


A circadian clock in the sinus node mediates day-night rhythms in Hcn4 and heart rate

D’Souza A, Wang Y, Anderson C, Bucchi A, Barsucotti M, Olieslagers S, Mesirca P, Johnsen AB, Mastitskaya S, Ni H, Zhang Y, Black N, Cox C, Wegner S, Bano- Otalora B, Petit C, Gill E, Logantha SJ, Dobrzynski H, Ashton N, Hart G, Zhang R, Zhang H, Cartwright EJ, Wisloff U, Mangoni ME, Da Costa Martins P, Piggins HD, DiFrancesco D, Boyett MR. Heart Rhythm. 2020 Nov 27:S1547-5271(20)31130-9. doi: 10.1016/j.hrthm.2020.11.026. Epub ahead of print. PMID: 33278629.


High content screening identifies licoisoflavone A as a bioactive compound of Tongmaiyangxin Pills to restrain cardiomyocyte hypertrophy via activating Sirt3

D’Souza A, Wang Y, Anderson C, Bucchi A, Barsucotti M, Olieslagers S, Mesirca P, Johnsen AB, Mastitskaya S, Ni H, Zhang Y, Black N, Cox C, Wegner S, Bano- Otalora B, Petit C, Gill E, Logantha SJ, Dobrzynski H, Ashton N, Hart G, Zhang R, Zhang H, Cartwright EJ, Wisloff U, Mangoni ME, Da Costa Martins P, Piggins HD, DiFrancesco D, Boyett MR. Heart Rhythm. 2020 Nov 27:S1547-5271(20)31130-9. doi: 10.1016/j.hrthm.2020.11.026. Epub ahead of print. PMID: 33278629.


Human iPSC modelling of a familial form of atrial fibrillation reveals a gain of function of If and ICaL in patient-derived cardiomyocytes

Benzoni P, Campostrini G, Landi S, Bertini V, Marchina E, Iascone M, Ahlberg G, Olesen MS, Crescini E, Mora C, Bisleri G, Muneretto C, Ronca R, Presta M, Poliani PL, Piovani G, Verardi R, Di Pasquale E, Consiglio A, Raya A, Torre E, Lodrini AM, Milanesi R, Rocchetti M, Baruscotti M, DiFrancesco D, Memo M, Barbuti A, Dell’Era P. Cardiovasc Res. 2020 May 1;116(6):1147-1160. doi: 10.1093/cvr/cvz217. PMID: 31504264; PMCID: PMC7177512.


Generation of the induced human pluripotent stem cell lines CSSi009-A from a patient with a GNB5 pathogenic variant, and CSSi010-A from a CRISPR/Cas9 engineered GNB5 knock-out human cell line.

Malerba N, Benzoni P, Squeo GM, Milanesi R, Giannetti F, Sadleir LG, Poke G, Augello B, Croce AI, Barbuti A, Merla G. Stem Cell Res. 2019 Oct;40:101547. doi: 10.1016/j.scr.2019.101547. Epub 2019 Aug 22. PMID: 31479876.


Optical Investigation of Action Potential and Calcium Handling Maturation of hiPSC-Cardiomyocytes on Biomimetic Substrates

Pioner JM, Santini L, Palandri C, Martella D, Lupi F, Langione M, Querceto S, Grandinetti B, Balducci V, Benzoni P, Landi S, Barbuti A, Ferrares Lupi F, Boarino L, Sartiani L, Tesi C, Mack DL, Regnier M, Cerbai E, Parmeggiani C, Poggesi C, Ferrantini C, Coppini R.. Int J Mol Sci. 2019 Aug 3;20(15):3799. doi: 10.3390/ijms20153799. PMID: 31382622; PMCID: PMC6695920.


HCN ion channels and accessory proteins in epilepsy: genetic analysis of a large cohort of patients and review of the literature.

DiFrancesco JC, Castellotti B, Milanesi R, Ragona F, Freri E, Canafoglia L, Franceschetti S, Ferrarese C, Magri S, Taroni F, Costa C, Labate A, Gambardella A, Solazzi R, Binda A, Rivolta I, Di Gennaro G, Casciato S, D’Incerti L, Barbuti A, DiFrancesco D, Granata T, Gellera C. Epilepsy Res. 2019 Jul;153:49-58. doi: 10.1016/j.eplepsyres.2019.04.004. Epub 2019 Apr 8. PMID: 30986657.


Screening and Identification of Cardioprotective Compounds From Wenxin Keli by Activity Index Approach and in vivo Zebrafish Model

Liu H, Chen X, Zhao X, Zhao B, Qian K, Shi Y, Baruscotti M, Wang Y.. Front Pharmacol. 2018 Nov 13;9:1288. doi: 10.3389/fphar.2018.01288. PMID: 30483130; PMCID: PMC6243390.


HCN1 mutation spectrum: from neonatal epileptic encephalopathy to benign generalized epilepsy and beyond

Marini C, Porro A, Rastetter A, Dalle C, Rivolta I, Bauer D, Oegema R, Nava C, Parrini E, Mei D, Mercer C, Dhamija R, Chambers C, Coubes C, Thévenon J, Kuentz P, Julia S, Pasquier L, Dubourg C, Carré W, Rosati A, Melani F, Pisano T, Giardino M, Innes AM, Alembik Y, Scheidecker S, Santos M, Figueiroa S, Garrido C, Fusco C, Frattini D, Spagnoli C, Binda A, Granata T, Ragona F, Freri E, Franceschetti S, Canafoglia L, Castellotti B, Gellera C, Milanesi R, Mancardi MM, Clark DR, Kok F, Helbig KL, Ichikawa S, Sadler L, Neupauerová J, Laššuthova P, Šterbová K, Laridon A, Brilstra E, Koeleman B, Lemke JR, Zara F, Striano P, Soblet J, Smits G, Deconinck N, Barbuti A, DiFrancesco D, LeGuern E, Guerrini R, Santoro B, Hamacher K, Thiel G, Moroni A, DiFrancesco JC, Depienne C.. Brain. 2018 Nov 1;141(11):3160-3178. doi: 10.1093/brain/awy263. PMID: 30351409.


A Loss-of-Function HCN4 Mutation Associated With Familial Benign Myoclonic Epilepsy in Infancy Causes Increased Neuronal Excitability.

Campostrini G, DiFrancesco JC, Castellotti B, Milanesi R, Gnecchi-Ruscone T, Bonzanni M, Bucchi A, Baruscotti M, Ferrarese C, Franceschetti S, Canafoglia L, Ragona F, Freri E, Labate A, Gambardella A, Costa C, Gellera C, Granata T, Barbuti A, DiFrancesco D. Front Mol Neurosci. 2018 Aug 6;11:269. doi: 10.3389/fnmol.2018.00269. PMID: 30127718; PMCID: PMC6089338.


Modulation of the intrinsic neuronal excitability by multifunctional liposomes tailored for the treatment of Alzheimer’s disease.

Binda A, Panariti A, Barbuti A, Murano C, Dal Magro R, Masserini M, Re F, Rivolta I. Int J Nanomedicine. 2018 Jul 11;13:4059-4071. doi: 10.2147/IJN.S161563. PMID: 30034232; PMCID: PMC6047604.


A novel de novo HCN1 loss-of-function mutation in genetic generalized epilepsy causing increased neuronal excitability.

Bonzanni M, DiFrancesco JC, Milanesi R, Campostrini G, Castellotti B, Bucchi A, Baruscotti M, Ferrarese C, Franceschetti S, Canafoglia L, Ragona F, Freri E, Labate A, Gambardella A, Costa C, Rivolta I, Gellera C, Granata T, Barbuti A, DiFrancesco D. A novel de novo HCN1 loss-of-function mutation in genetic generalized epilepsy causing increased neuronal excitability. Neurobiol Dis. 2018 Oct;118:55-63. doi: 10.1016/j.nbd.2018.06.012. Epub 2018 Jun 21. PMID: 29936235.


A synthetic peptide that prevents cAMP regulation in mammalian hyperpolarization- activated cyclic nucleotide-gated (HCN) channels.

Saponaro A, Cantini F, Porro A, Bucchi A, DiFrancesco D, Maione V, Donadoni C, Introini B, Mesirca P, Mangoni ME, Thiel G, Banci L, Santoro B, Moroni A. Elife. 2018 Jun 20;7:e35753. doi: 10.7554/eLife.35753. PMID: 29923826; PMCID: PMC6023613.


Effects of repeated sprints training on fracture risk-associated miRNA

Sansoni V, Perego S, Vernillo G, Barbuti A, Merati G, La Torre A, Banfi G, Lombardi G. Oncotarget. 2018 Apr 6;9(26):18029-18040. doi: 10.18632/oncotarget.24707. PMID: 29719588; PMCID: PMC5915055.


Generation of human induced pluripotent stem cells (EURACi001-A, EURACi002-A, EURACi003-A) from peripheral blood mononuclear cells of three patients carrying mutations in the CAV3 gene.

Meraviglia V, Benzoni P, Landi S, Murano C, Langione M, Motta BM, Baratto S, Silipigni R, Di Segni M, Pramstaller PP, DiFrancesco D, Gazzerro E, Barbuti A, Rossini A. Stem Cell Res. 2018 Mar;27:25-29. doi: 10.1016/j.scr.2017.12.012. Epub 2017 Dec 18. PMID: 29304398.


Preferential myofibroblast differentiation of cardiac mesenchymal progenitor cells in the presence of atrial fibrillation.

Gambini E, Perrucci GL, Bassetti B, Spaltro G, Campostrini G, Lionetti MC, Pilozzi A, Martinelli F, Farruggia A, DiFrancesco D, Barbuti A, Pompilio G. Transl Res. 2018 Feb;192:54-67. doi: 10.1016/j.trsl.2017.11.003. Epub 2017 Nov 27. PMID: 29245016.


Mammalian γ2 AMPK regulates intrinsic heart rate.

Yavari A, Bellahcene M, Bucchi A, Sirenko S, Pinter K, Herring N, Jung JJ, Tarasov KV, Sharpe EJ, Wolfien M, Czibik G, Steeples V, Ghaffari S, Nguyen C, Stockenhuber A, Clair JRS, Rimmbach C, Okamoto Y, Yang D, Wang M, Ziman BD, Moen JM, Riordon DR, Ramirez C, Paina M, Lee J, Zhang J, Ahmet I, Matt MG, Tarasova YS, Baban D, Sahgal N, Lockstone H, Puliyadi R, de Bono J, Siggs OM, Gomes J, Muskett H, Maguire ML, Beglov Y, Kelly M, Dos Santos PPN, Bright NJ, Woods A, Gehmlich K, Isackson H, Douglas G, Ferguson DJP, Schneider JE, Tinker A, Wolkenhauer O, Channon KM, Cornall RJ, Sternick EB, Paterson DJ, Redwood CS, Carling D, Proenza C, David R, Baruscotti M, DiFrancesco D, Lakatta EG, Watkins H, Ashrafian H. Nat Commun. 2017 Nov 2;8(1):1258. doi: 10.1038/s41467-017-01342-5. PMID: 29097735; PMCID: PMC5668267.


The expression of the rare caveolin-3 variant T78M alters cardiac ion channels function and membrane excitability.

Campostrini G, Bonzanni M, Lissoni A, Bazzini C, Milanesi R, Vezzoli E, Francolini M, Baruscotti M, Bucchi A, Rivolta I, Fantini M, Severi S, Cappato R, Crotti L, J Schwartz P, DiFrancesco D, Barbuti A. Cardiovasc Res. 2017 Aug 1;113(10):1256-1265. doi: 10.1093/cvr/cvx122. PMID: 28898996; PMCID: PMC5852518.


MiR-320a as a Potential Novel Circulating Biomarker of Arrhythmogenic CardioMyopathy.

Sommariva E, D’Alessandra Y, Farina FM, Casella M, Cattaneo F, Catto V, Chiesa M, Stadiotti I, Brambilla S, Dello Russo A, Carbucicchio C, Vettor G, Riggio D, Sandri MT, Barbuti A, Vernillo G, Muratori M, Dal Ferro M, Sinagra G, Moimas S, Giacca M, Colombo GI, Pompilio G, Tondo C. Sci Rep. 2017 Jul 6;7(1):4802. doi: 10.1038/s41598-017-05001-z. PMID: 28684747; PMCID: PMC5500514.


A gain-of-function mutation in the cardiac pacemaker HCN4 channel increasing cAMP sensitivity is associated with familial Inappropriate Sinus Tachycardia.

Baruscotti M, Bucchi A, Milanesi R, Paina M, Barbuti A, Gnecchi-Ruscone T, Bianco E, Vitali-Serdoz L, Cappato R, DiFrancesco D. Eur Heart J. 2017 Jan 21;38(4):280-288. doi: 10.1093/eurheartj/ehv582. PMID: 28182231.


Bone turnover response is linked to both acute and established metabolic changes in ultra-marathon runners.

Sansoni V, Vernillo G, Perego S, Barbuti A, Merati G, Schena F, La Torre A, Banfi G, Lombardi G. Endocrine. 2017 Apr;56(1):196-204. doi: 10.1007/s12020-016-1012-8. Epub 2016 Jul 15. PMID: 27422791.


Plasminogen activator inhibitor-1 as a marker of cardiovascular response in professional mountain ultra-marathon runners.

Lombardi G, Vernillo G, Sansoni V, Perego S, Barbuti A, Merati G, Schena F, Banfi G, La Torre A. P Clin Chem Lab Med. 2017 Jan 1;55(1):e7-e9. doi: 10.1515/cclm-2016-0295. PMID: 27276530.


Human derived cardiomyocytes: A decade of knowledge after the discovery of induced pluripotent stem cells.

Barbuti A, Benzoni P, Campostrini G, Dell’Era P. Dev Dyn. 2016 Dec;245(12):1145-1158. doi: 10.1002/dvdy.24455. Epub 2016 Oct 7. PMID: 27599668.


INaP selective inhibition reverts precocious inter- and motorneurons hyperexcitability in the Sod1-G93R zebrafish ALS model.

Benedetti L, Ghilardi A, Rottoli E, De Maglie M, Prosperi L, Perego C, Baruscotti M, Bucchi A, Del Giacco L, Francolini M. Sci Rep. 2016 Apr 15;6:24515. doi: 10.1038/srep24515. PMID: 27079797; PMCID: PMC4832213.


Current understanding of the pathophysiological mechanisms responsible for inappropriate sinus tachycardia: role of the If “funny” current.

Baruscotti M, Bianco E, Bucchi A, DiFrancesco D. J Interv Card Electrophysiol. 2016 Jun;46(1):19-28. doi: 10.1007/s10840-015-0097-y. Epub 2016 Jan 18. PMID: 26781742.