Several strategies are available for genomic editing, including CRISPR/Cas9, Cre/LoxP, DNA/ribonucleoprotein electroporation, retro/lentiviral vector infection and microinjection. With these, it is possible to engineer cellular and animal models, like embryonic stem cells, induced pluripotent stem cells, hematopoietic cells, neurons, zebrafish embryos and larvae, in utero embryos, organoids and tumoroids.

The zebrafish platform (Pistocchi’s lab, Persani’s lab, Vitale’s lab, Bonomi’s lab, Marozzi’s lab) has a long standing experience in the use of zebrafish as an experimental model. Our basic research cover zebrafish gene clone, gene expression analysis, gene editing, microinjection, genotyping, health monitoring and high content imaging-based phenotypic assays. Indeed, for identifying small molecules with biological activity, high-throughput analysis easily provides information on tissue specificity, toxicity, and bioavailability.